Purification and characterization of 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase involved in ergothioneine utilization in <i>Burkholderia</i> sp. HME13-Reference-Cited by-同舟云学术

Purification and characterization of 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase involved in ergothioneine utilization in Burkholderia sp. HME13

Published:2023-09-27 Issue:1 Volume:88 Page:74-78
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Muramatsu Hisashi¹,Inouchi Daisuke²,Yamada Masaaki³,Koujitani Akihito³,Maguchi Hiroki³,Kato Shin-ichiro¹

Affiliation:

1. Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University , B200 Monobe, Nankoku, Kochi , Japan

2. Faculty of Agriculture and Marine Science, Kochi University , B200 Monobe, Nankoku, Kochi , Japan

3. Graduate School of Integrated Arts and Sciences, Kochi University , B200 Monobe, Nankoku, Kochi , Japan

Abstract

ABSTRACT Recombinant 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase (ErtC) derived from Burkholderia sp. HME13 was purified to homogeneity. Here, ErtC's kinetic parameters, optimum reaction temperature and pH, and stability at varying temperatures and pH and the effects of various additives on ErtC activity were determined. Real-time polymerase chain reaction and enzyme assays suggested that ergothioneine induced the expression of ertC.

Funder

JSPS

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbad139/52248720/zbad139.pdf

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