Heterologous expression of the lectin CmRlec from <i>Cordyceps militaris</i> (Cordycipitaceae, Ascomycota) in <i>Escherichia coli</i>-Reference-Cited by-同舟云学术

Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli

Published:2023-04-18 Issue:7 Volume:87 Page:742-746
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Ono Akiko¹²,Zhang Jili¹,Tanaka Yuki¹^ORCID,Sato Chihiro¹,Yoda Akiyoshi¹²,Ogata Makoto³,Nomura Takahito¹,Suzuki Tomohiro¹^ORCID

Affiliation:

1. Center for Bioscience Research and Education, Utsunomiya University , 350 Mine-machi, Utsunomiya, Tochigi , Japan

2. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology , 3-5-8 Saiwai-cho, Fuchu, Tokyo , Japan

3. Faculty of Food and Agricultural Sciences, Fukushima University , 1 Kanayagawa, Fukushima, Fukushima , Japan

Abstract

ABSTRACT Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin.

Funder

Grant-in-Aid for Scientific Research

Hokuto Foundation for Bioscience

Sasakawa Scientific Research

Japan Science Society

JSPS

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbad045/50158246/zbad045.pdf