METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue-Reference-Cited by-同舟云学术

METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue

Published:2020-06-23 Issue:14 Volume:48 Page:8022-8034
ISSN:0305-1048
Container-title:Nucleic Acids Research
language:en
Short-container-title:

Author:

Laptev Ivan¹²³,Shvetsova Ekaterina⁴,Levitskii Sergey⁵,Serebryakova Marina¹³,Rubtsova Maria¹²^ORCID,Zgoda Victor⁶,Bogdanov Alexey²³^ORCID,Kamenski Piotr⁵,Sergiev Petr¹²³⁷^ORCID,Dontsova Olga¹²³⁸

Affiliation:

1. Center of Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo, Moscow 143028, Russia

2. Department of Chemistry, Lomonosov Moscow State University, Moscow 119992, Russia

3. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia

4. Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119992, Russia

5. Faculty of Biology, Lomonosov Moscow State University, Moscow 119992, Russia

6. Institute of Biomedical Chemistry, Moscow 119435, Russia

7. Institute of Functional Genomics, Lomonosov Moscow State University, Moscow 119992, Russia

8. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia

Abstract

AbstractMammalian mitochondrial ribosomes contain a set of modified nucleotides, which is distinct from that of the cytosolic ribosomes. Nucleotide m4C840 of the murine mitochondrial 12S rRNA is equivalent to the dimethylated m4Cm1402 residue of Escherichia coli 16S rRNA. Here we demonstrate that mouse METTL15 protein is responsible for the formation of m4C residue of the 12S rRNA. Inactivation of Mettl15 gene in murine cell line perturbs the composition of mitochondrial protein biosynthesis machinery. Identification of METTL15 interaction partners revealed that the likely substrate for this RNA methyltransferase is an assembly intermediate of the mitochondrial small ribosomal subunit containing an assembly factor RBFA.

Funder

Russian Science Foundation

Skolkovo Institute of Science and Technology

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

http://academic.oup.com/nar/article-pdf/48/14/8022/34131366/gkaa522.pdf

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