Characterization of a sweet basil acyltransferase involved in eugenol biosynthesis

Author:

Dhar Niha1ORCID,Sarangapani Sreelatha1,Reddy Vaishnavi Amarr1ORCID,Kumar Nadimuthu1,Panicker Deepa12,Jin Jingjing13ORCID,Chua Nam-Hai14ORCID,Sarojam Rajani1ORCID

Affiliation:

1. Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore

2. Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore

3. China Tobacco Gene Research Centre, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China

4. Laboratory of Plant Molecular Biology, The Rockefeller University, New York, NY, USA

Abstract

Abstract Sweet basil (Ocimum basilicum) plants produce its characteristic phenylpropene-rich essential oil in specialized structures known as peltate glandular trichomes (PGTs). Eugenol and chavicol are the major phenylpropenes produced by sweet basil varieties whose synthetic pathways are not fully elucidated. Eugenol is derived from coniferyl acetate by a reaction catalysed by eugenol synthase. An acyltransferase is proposed to convert coniferyl alcohol to coniferyl acetate which is the first committed step towards eugenol synthesis. Here, we perform a comparative next-generation transcriptome sequencing of different tissues of sweet basil, namely PGT, leaf, leaf stripped of PGTs (leaf–PGT), and roots, to identify differentially expressed transcripts specific to PGT. From these data, we identified a PGT-enriched BAHD acyltransferase gene ObCAAT1 and functionally characterized it. In vitro coupled reaction of ObCAAT1 with eugenol synthase in the presence of coniferyl alcohol resulted in eugenol production. Analysis of ObCAAT1-RNAi transgenic lines showed decreased levels of eugenol and accumulation of coniferyl alcohol and its derivatives. Coniferyl alcohol acts as a common substrate for phenylpropene and lignin biosynthesis. No differences were found in total lignin content of PGTs and leaves of transgenic lines, indicating that phenylpropene biosynthesis is not coupled to lignification in sweet basil.

Funder

National Research Foundation Singapore

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

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