Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning-Reference-Cited by-同舟云学术

Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning

Published:2024-05-10 Issue:7 Volume:88 Page:784-788
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Kuzuhara Taiki¹²,Monden Kota¹²,Hachiya Takushi¹²³^ORCID,Nakagawa Tsuyoshi¹²³

Affiliation:

1. Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University , Matsue , Japan

2. Graduate School of Natural Science and Technology, Shimane University , Matsue , Japan

3. Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University , Matsue , Japan

Abstract

ABSTRACT Gateway cloning is a useful technology for the simple and reliable preparation of various construct in many organisms. However, there is a problem regarding the negative control construct in the Gateway cloning system. In this study, we developed the pENTR-NeCo-lacZα vector system to create an empty vector that can be used as a negative control construct in Gateway cloning.

Funder

Japan Society for the Promotion of Science

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbae061/58084748/zbae061.pdf

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