Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins-Reference-Cited by-同舟云学术

Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins

Published:2024-03-05 Issue:5 Volume:88 Page:555-560
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Kuroiwa Fu¹,Suda Hiraku¹,Yabuki Maho¹,Atsuzawa Kimie²,Yamaguchi Haruhiko³,Toyota Masatsugu¹^ORCID,Kaneko Yasuko⁴,Yamashita Satoshi⁵^ORCID,Takahashi Seiji⁶,Tozawa Yuzuru¹^ORCID

Affiliation:

1. Graduate School of Science and Engineering, Saitama University , Saitama , Japan

2. Comprehensive Analysis Center for Science, Saitama University , Saitama , Japan

3. Sumitomo Rubber Industries Ltd. , Kobe, Hyogo , Japan

4. Department of Natural Science in the Faculty of Education, Saitama University , Saitama , Japan

5. Graduate School of Natural Science and Technology, Kanazawa University, Kakuma , Kanazawa , Japan

6. Graduate School of Engineering, Tohoku University , Sendai, Miyagi , Japan

Abstract

ABSTRACT Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and lipid droplets are limited. We have succeeded in establishing a system in which artificially prepared lipid monolayer particles are added to a cell-free translation system to confirm the properties of proteins that specifically bind to lipid monolayers in a translation-coupled manner.

Funder

Japan Society for the Promotion of Science

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbae026/57139824/zbae026.pdf

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