Effects of Laboratory Grade Limonene and a Commercial Limonene-Based Insecticide on Haematobia irritans irritans (Muscidae: Diptera): Deterrence, Mortality, and Reproduction

Abstract

Abstract The horn fly, Haematobia irritans irritans (L.) (Diptera: Muscidae), is an important and cosmopolitan blood feeding ectoparasite of cattle. Resistance to conventional insecticides is increasingly problematic and alternative pesticides, including natural products, are being investigated. Limonene is a cyclic monoterpene repellent to some insects that occurs in citrus fruit rinds and in other plants. We assessed laboratory grade limonene and a commercial product, Orange Guard (5.8% AI limonene), against H. irritans irritans in terms of their contact effects upon contact on egg mortality, adults, and larval and pupal development; adult repellency as well as sublethal and fumigation effects. Egg viability declined when they were exposed to Orange Guard at concentrations of 1.45%, 2.9%, and 5.8% whereas laboratory grade limonene at 5.8% and 11.6% was ovicidal. Contact exposure of adult H. irritans irritans to 5.8% laboratory grade limonene and 2.9% Orange Guard caused up to 100 and 88% knockdown (immobilization), respectively. At higher concentrations, laboratory grade limonene and Orange Guard resulted in less, and often shorter periods of knockdown. Although direct contact of 2.9 and 5.8% laboratory grade limonene caused mortality it was negligible when flies were sprayed directly with undiluted Orange Guard. Female H. irritans irritans exposed to sublethal concentrations of Orange Guard did not reduce the numbers of eggs produced, but the undiluted product reduced egg hatchability. Interestingly, limonene and Orange Guard attracted adult H. irritans irritans at concentrations <0.1%. We suggest that the attractancy of unformulated pure limonene might be useful for trapping H. irritans irritans adults.