Baseline micronucleus frequencies and 60Co cytokinesis-block micronucleus assay dose–response curve for biodosimetry in Vietnam-Reference-Cited by-同舟云学术

Baseline micronucleus frequencies and 60Co cytokinesis-block micronucleus assay dose–response curve for biodosimetry in Vietnam

Published:2023-10-07 Issue: Volume: Page:
ISSN:0144-8420
Container-title:Radiation Protection Dosimetry
language:en
Short-container-title:

Author:

Pham Ngoc Duy¹,Tran Thanh Mai¹,Anderson Donovan²,Che Quang Tuan¹,Pham Ho Thuat Khoa¹

Affiliation:

1. Dalat Nuclear Research Institute Radiation Technology and Biotechnology Center, , No. 01, Nguyen Tu Luc Street, Dalat City, Lamdong Province 66000, Vietnam

2. Hirosaki University Institute of Radiation Emergency Medicine, , 66-1, Hon-cho, Hirosaki-shi, Aomori 036-8564 , Japan

Abstract

Abstract This study aims to establish baseline micronucleus (MN) frequencies from various populations of residents in Vietnam and develop a 60Co dose–response curve for the cytokinesis-block micronucleus (CBMN) assay. Blood samples were exposed in vitro to a 60Co source at a dose rate of 275 mGy per min in a range of 0.1 to 4.0 Gy. MN background frequencies were 4.5 ± 3.2, 7.3 ± 4.6, 7.0 ± 3.8 and 13.1 ± 6.7 in 1000 binucleated (BN) cells for 96 healthy donors, 22 male radiation workers and 12 breast cancer patients, respectively. Blood samples from three healthy donors were used to generate the MN dose–response curve: y = C + (0.0496 ± 0.0069)D + (0.0143 ± 0.0026)D2. This curve was verified through an inter-laboratory comparison (RENEB ILC 2021). Our findings highlight the significance of the CBMN assay as an additional essential tool for biodosimetry in Vietnam.

Funder

International Atomic Energy Agency

Publisher

Oxford University Press (OUP)

Subject

Public Health, Environmental and Occupational Health,Radiology, Nuclear Medicine and imaging,General Medicine,Radiation,Radiological and Ultrasound Technology

Link

https://academic.oup.com/rpd/advance-article-pdf/doi/10.1093/rpd/ncad266/56453699/ncad266.pdf

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1. Correction to: Baseline micronucleus frequencies and 60Co cytokinesis-block micronucleus assay dose–response curve for biodosimetry in Vietnam;Radiation Protection Dosimetry;2024-01-30