Chronic skin damage induces small intestinal damage via IL-13-induced apoptosis
Author:
Tanemoto Rina1, Higashiyama Masaaki1ORCID, Tomioka Akira1, Ito Suguru1, Mizoguchi Akinori1, Nishii Shin1, Inaba Kenichi1, Wada Akinori1, Sugihara Nao1, Hanawa Yoshinori1, Horiuchi Kazuki1, Okada Yoshikiyo1, Kurihara Chie1, Akita Yoshihiro1, Narimatsu Kazuyuki1, Komoto Shunsuke1, Tomita Kengo1, Satoh Takahiro2, Tsuda Hitoshi3ORCID, Hokari Ryota1
Affiliation:
1. Department of Gastroenterology, National Defense Medical College , Saitama , Japan 2. Department of Dermatology, National Defense Medical College , Saitama , Japan 3. Department of Basic Pathology, National Defense Medical College , Saitama , Japan
Abstract
Abstract
The gut–skin axis has recently been widely recognized, and both the gut and skin have been found to affect each other through a bidirectional connection; however, the precise mechanisms remain to be elucidated. Therefore, we aimed to investigate the effects of chronic skin damage (CSD) on mouse intestines. Following the CSD model, 4% sodium dodecyl sulfate was applied to the back-shaved murine skin six times for 2 weeks after tape stripping. The small and large intestines were analyzed histologically and immunologically, respectively. Intestinal permeability was measured using fluorescein isothiocyanate-conjugated-dextran. The role of interleukin-13 (IL-13) in the ileum was investigated using an anti-IL-13 antibody. Apoptotic intestinal cells were analyzed using TUNEL staining. Villus atrophy was observed in the small intestine in the CSD model, along with increased permeability. Mast cells, but not T cells, eosinophils, or innate lymph cell-2, were increased in the intestinal mucosa. However, no significant changes were observed in the large intestine. mRNA expression of IL-13 was increased only in the ileum of the CSD model. Apoptotic intestinal epithelial cells were significantly increased in the ileum of the CSD model. Administration of an anti-IL-13 antibody ameliorated the intestinal damage caused by CSD, along with decreased apoptotic cells and mast cell infiltration. Skin damage causes morphological changes in the small intestine, accompanied by increased intestinal permeability, possibly through the IL-13-induced apoptosis of mast cells in the epithelium. Surfactant-mediated mechanical skin damage can cause a leaky gut.
Funder
National Defense Medical College Ministry of Health, Labour, and Welfare, Japan
Publisher
Oxford University Press (OUP)
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