Affiliation:
1. Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine , Shanghai 200437 , China
2. Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine, Tongji University , Shanghai 200443 , China
3. Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine , Shanghai 201203 , China
Abstract
Abstract
Psoriasis is a chronic immune-mediated recurrent skin disease causing systemic damage. Increased angiogenesis has been reported to participate in the progression of psoriasis. However, angiogenesis-related genes (ARGs) in psoriasis have not been systematically elucidated. Therefore, we aim to identify potential biomarkers and subtypes using two algorithmsr. Transcriptome sequencing data of patients with psoriasis were obtained, in which differentially expressed genes were assessed by principal component analysis. A diagnostic model was developed using random forest algorithm and validated by receiver operating characteristic (ROC) curves. Subsequently, we performed consensus clustering to calculate angiogenesis-associated molecular subtypes of psoriasis. Additionally, a correlation analysis was conducted between ARGs and immune cell infiltration. Finally, validation of potential ARG genes was performed by quantitative real-time PCR (qRT-PCR). We identified 29 differentially expressed ARGs, including 13 increased and 16 decreased. Ten ARGs, CXCL8, ANG, EGF, HTATIP2, ANGPTL4, TNFSF12, RHOB, PML, FOXO4, and EMCN were subsequently sifted by the diagnostic model based on a random forest algorithm. Analysis of the ROC curve (area under the curve [AUC] = 1.0) indicated high diagnostic performance in internal validation. The correlation analysis suggested that CXCL8 has a high positive correlation with neutrophil (R =0.8, P < 0.0001) and interleukins pathway (R = 0.79, P < 0.0001). Furthermore, two ARG-mediated subtypes were obtained, indicating potential heterogeneity. Finally, the qRT-PCR demonstrated that the mRNA expression levels of CXCL8 and ANGPTL4 were elevated in psoriasis patients, with a reduced expression of EMCN observed. The current paper indicated potential ARG-related biomarkers of psoriasis, including CXCL8, ANGPTL4, and EMCN, with two molecular subtypes.
Funder
National Natural Science Foundation of China
Shanghai Science and Technology Committee
The Shanghai Development Office of TCM
Shanghai Dermatology Hospital
Shanghai Dermatology Research Center
Shanghai Municipal Health Commission Health Industry Clinical Research Special Project
Shanghai Science and Technology Development Funds
Shanghai Shenkang Second Round Three Year Action Plan for Clinical Trial Program
Hospital Management Program of Shanghai Hospital Association
Shanghai Health Bureau Clinical Research Program
China Fund for Medical Equipment
China YMIOO Program
Clinical Transformation Incubation Program in Hospital
Publisher
Oxford University Press (OUP)