Bacterial communities of Antarctic lichens explored by gDNA and cDNA 16S rRNA gene amplicon sequencing

Author:

Woltyńska Aleksandra1,Gawor Jan1,Olech Maria A2,Górniak Dorota3,Grzesiak Jakub1ORCID

Affiliation:

1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Pawińskiego 5A, 02-106 Warszawa, Poland

2. Institute of Botany, Jagiellonian University , Gronostajowa 3, 30-387 Krakow, Poland

3. Department of Microbiology and Mycology, University of Warmia and Mazury in Olsztyn , Oczapowskiego 1a, 10-719 Olsztyn, Poland

Abstract

Abstract Recently, lichens came once more into the scientific spotlight due to their unique relations with prokaryotes. Several temperate region lichen species have been thoroughly explored in this regard yet, the information on Antarctic lichens and their associated bacteriobiomes is somewhat lacking. In this paper, we assessed the phylogenetic structure of the whole and active fractions of bacterial communities housed by Antarctic lichens growing in different environmental conditions by targeted 16S rRNA gene amplicon sequencing. Bacterial communities associated with lichens procured from a nitrogen enriched site were very distinct from the communities isolated from lichens of a nitrogen depleted site. The former were characterized by substantial contributions of Bacteroidetes phylum members and the elusive Armatimonadetes. At the nutrient-poor site the lichen-associated bacteriobiome structure was unique for each lichen species, with chlorolichens being occupied largely by Proteobacteria. Lichen species with a pronounced discrepancy in diversity between the whole and active fractions of their bacterial communities had the widest ecological amplitude, hinting that the nonactive part of the community is a reservoir of latent stress coping mechanisms. This is the first investigation to make use of targeted metatranscriptomics to infer the bacterial biodiversity in Antarctic lichens.

Funder

National Science Centre, Poland

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Ecology,Microbiology

Reference59 articles.

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