Succession and potential role of bacterial communities during Pleurotus ostreatus production

Author:

Bánfi Renáta1,Pohner Zsuzsanna12,Szabó Attila1,Herczeg Gábor3,Kovács Gábor M4ORCID,Nagy Adrienn5,Márialigeti Károly1,Vajna Balázs1ORCID

Affiliation:

1. Department of Microbiology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary

2. Institute for Soil Sciences and Agricultural Chemistry, Centre for Agricultural Research, Herman Ottó út 15, H-1022 Budapest, Hungary

3. Department of Systematic Zoology and Ecology, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary

4. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117 Budapest, Hungary

5. Pilze-Nagy Ltd., Talfája 50., H-6000 Kecskemét, Hungary

Abstract

ABSTRACT There is an increasing interest in studying bacterial-fungal interactions (BFIs), also the interactions of Pleurotus ostreatus, a model white-rot fungus and important cultivated mushroom. In Europe, P. ostreatus is produced on a wheat straw-based substrate with a characteristic bacterial community, where P. ostreatus is exposed to the microbiome during substrate colonisation. This study investigated how the bacterial community structure was affected by the introduction of P. ostreatus into the mature substrate. Based on the results obtained, the effect of the presence and absence of this microbiome on P. ostreatus production in an experimental cultivation setup was determined. 16S rRNA gene-based terminal restriction fragment length polymorphism (T-RFLP) and amplicon sequencing revealed a definite succession of the microbiome during substrate colonisation and fruiting body production: a sharp decrease in relative abundance of Thermus spp. and Actinobacteria, and the increasing dominance of Bacillales and Halomonas spp. The introduced experimental cultivation setup proved the protective role of the microbial community against competing fungi without affecting P. ostreatus growth. We could also demonstrate that this effect could be attributed to both living microbes and their secreted metabolites. These findings highlight the importance of bacterial-fungal interactions during mushroom production.

Funder

Hungarian Scientific Research Fund

European Social Fund

National Research, Development and Innovation Fund

Hungarian Academy of Sciences

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Ecology,Microbiology

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