The Fate of Mitochondria in Ibex-hirus Reconstructed Early Embryos-Reference-Cited by-同舟云学术

The Fate of Mitochondria in Ibex-hirus Reconstructed Early Embryos

Published:2004-05-01 Issue:5 Volume:36 Page:371-374
ISSN:1745-7270
Container-title:Acta Biochimica et Biophysica Sinica
language:en
Short-container-title:

Author:

Jiang Yan¹,Liu Shu-Zhen¹,Zhang Yan-Ling¹,Jiang Man-Xi¹,Sun Qing-Yuan¹,Chen Da-Yuan¹

Affiliation:

1. State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Sciences Beijing 100080, China

Abstract

Abstract Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.

Publisher

China Science Publishing & Media Ltd.

Subject

General Medicine,Biochemistry,Biophysics

Link

http://academic.oup.com/abbs/article-pdf/36/5/371/28997666/36-5-371.pdf

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