Deep Imaging Analysis in VISUAL Reveals the Role of YABBY Genes in Vascular Stem Cell Fate Determination

Author:

Nurani Alif Meem1,Ozawa Yasuko1,Furuya Tomoyuki1,Sakamoto Yuki23,Ebine Kazuo45,Matsunaga Sachihiro3,Ueda Takashi45ORCID,Fukuda Hiroo1,Kondo Yuki1

Affiliation:

1. Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan

2. Department of Biological Sciences, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043 Japan

3. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 Japan

4. Division of Cellular Dynamics, National Institute for Basic Biology, 38 Nishigounaka, Myodaiji, Okazaki, Aichi, 444-8585 Japan

5. Department of Basic Biology, The Graduate University for Advanced Studies (SOKENDAI), 38 Nishigounaka, Myodaiji, Okazaki, Aichi, 444-8585 Japan

Abstract

Abstract Stem cells undergo cell division and differentiation to ensure organized tissue development. Because plant cells are immobile, plant stem cells ought to decide their cell fate prior to differentiation, to locate specialized cells in the correct position. In this study, based on a chemical screen, we isolated a novel secondary cell wall indicator BF-170, which binds to lignin and can be used to image in vitro and in situ xylem development. Use of BF-170 to observe the vascular differentiation pattern in the in vitro vascular cell induction system, VISUAL, revealed that adaxial mesophyll cells of cotyledons predominantly generate ectopic xylem cells. Moreover, phloem cells are abundantly produced on the abaxial layer, suggesting the involvement of leaf adaxial–abaxial polarity in determining vascular cell fate. Analysis of abaxial polarity mutants highlighted the role of YAB3, an abaxial cell fate regulator, in suppressing xylem and promoting phloem differentiation on the abaxial domains in VISUAL. Furthermore, YABBY family genes affected in vivo vascular development during the secondary growth. Our results denoted the possibility that such mediators of spatial information contribute to correctly determine the cell fate of vascular stem cells, to conserve the vascular pattern of land plants.

Funder

Ministry of Education, Culture, Sports, Science, and Technology of Japan

Japan Society for the Promotion of Science

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Plant Science,Physiology,General Medicine

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