Prevalence and genetic characteristics offosB-positiveStaphylococcus aureusin duck farms in Guangdong, China in 2020

Author:

Hu Jianxin12,Chen Lin12,Li Guihua12,Pan Yu12,Lu Yixing12,Chen Jin2,Xiong Wenguang123ORCID,Zeng Zhenling123

Affiliation:

1. Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation College of Veterinary Medicine, South China Agricultural University , Guangzhou 510642 , China

2. National Risk Assessment Laboratory for Antimicrobial Resistant of Microorganisms in Animals, South China Agricultural University , Guangzhou 510642 , China

3. Guangdong Laboratory for Lingnan Modern Agriculture , Guangzhou , China

Abstract

AbstractObjectivesTo investigate the epidemiology of fosB-positive Staphylococcus aureus in waterfowl farms in the Pearl River tributaries in Guangdong Province, China in 2020.MethodsA total of 63 S. aureus were recovered from 315 samples collected from six duck farms and one goose farm. PFGE, WGS and analysis were performed on 19 fosB-positive S. aureus.ResultsThe fosfomycin resistance rate of the strains was as high as 52.4% (33/63), and 30.1% (19/63) of the strains carried fosB. Resistance gene prediction results showed that duck farm environment-derived strains contained the oxazolidinone drug resistance gene optrA. All fosB-positive S. aureus were MRSA and most of them were MDR, mainly ST9-t899 and ST164-t899. PFGE showed that fosB-positive S. aureus from humans and ducks could be clustered into the same clade. In addition, core-genome SNP analysis showed that clonal transmission of S. aureus occurred between humans and water. Pan-genome analysis showed that S. aureus had an open pangenome. The fosB gene was located on 2610–2615 bp plasmids, which all contained a broad host-range plasmid replication protein family 13. Small plasmids carrying the fosB gene could be found in different multilocus STs of S. aureus.ConclusionsThis study indicated that duck farms in Guangdong, China could be an important reservoir of fosB-positive S. aureus. The spread of drug-resistant bacteria in waterfowl farms requires further monitoring.

Funder

Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology,Microbiology (medical)

Reference38 articles.

1. Phosphonomycin, a new antibiotic produced by strains of Streptomyces;Hendlin;Science,1969

2. New microbiological aspects of fosfomycin;Díez-Aguilar;Rev Esp Quimioter,2019

3. Fosfomycin: uses and potentialities in veterinary medicine;Pérez;Open Vet J,2014

4. Emergence of fosfomycin-resistant isolates of Shiga-like toxin-producing Escherichia coli O26;Horii;Antimicrob Agents Chemother,1999

5. Structure and function of the Mur enzymes: development of novel inhibitors;Zoeiby A;Mol Microbiol,2003

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