Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei-Reference-Cited by-同舟云学术

Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei

Published:2020-11-27 Issue:1 Volume:11 Page:1-7
ISSN:2160-1836
Container-title:G3 Genes|Genomes|Genetics
language:en
Short-container-title:

Author:

Davey John W¹^ORCID,Catta-Preta Carolina M C¹²³^ORCID,James Sally¹,Forrester Sarah¹,Motta Maria Cristina M⁴⁵,Ashton Peter D¹,Mottram Jeremy C¹²^ORCID

Affiliation:

1. Department of Biology, University of York, York YO10 5DD, UK

2. York Biomedical Research Institute, University of York, York YO10 5DD, UK

3. Medicinal Chemistry Center (CQMED)/Structural Genomics Consortium, Universidade Estadual de Campinas, Campinas, São Paulo 13083-886, Brazil

4. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Departamento de Biologia Celular e Parasitologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

5. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens, Rio de Janeiro, RJ, Brazil

Abstract

Abstract Angomonas deanei is an endosymbiont-bearing trypanosomatid with several highly fragmented genome assemblies and unknown chromosome number. We present an assembly of the A. deanei nuclear genome based on Oxford Nanopore sequence that resolves into 29 complete or close-to-complete chromosomes. The assembly has several previously unknown special features; it has a supernumerary chromosome, a chromosome with a 340-kb inversion, and there is a translocation between two chromosomes. We also present an updated annotation of the chromosomal genome with 10,365 protein-coding genes, 59 transfer RNAs, 26 ribosomal RNAs, and 62 noncoding RNAs.

Funder

Wellcome Trust

Conselho Nacional de Desenvolvimento Científico e Tecnológico

CNPq

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ

Publisher

Oxford University Press (OUP)

Subject

Genetics (clinical),Genetics,Molecular Biology

Link

http://academic.oup.com/g3journal/article-pdf/11/1/1/36546181/jkaa018.pdf

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