A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience-Reference-Cited by-同舟云学术

A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience

Published:2021-01-01 Issue:1 Volume:11 Page:1-23
ISSN:2160-1836
Container-title:G3 Genes|Genomes|Genetics
language:en
Short-container-title:

Author:

Evans Cory J¹²,Olson John M¹,Mondal Bama Charan¹,Kandimalla Pratyush¹²,Abbasi Ariano¹,Abdusamad Mai M¹,Acosta Osvaldo¹,Ainsworth Julia A¹,Akram Haris M¹,Albert Ralph B¹,Alegria-Leal Elitzander¹,Alexander Kai Y¹,Ayala Angelica C¹,Balashova Nataliya S¹,Barber Rebecca M¹,Bassi Harmanjit¹,Bennion Sean P¹,Beyder Miriam¹,Bhatt Kush V¹,Bhoot Chinmay¹,Bradshaw Aaron W¹,Brannigan Tierney G¹,Cao Boyu¹,Cashell Yancey Y¹,Chai Timothy¹,Chan Alex W¹,Chan Carissa¹,Chang Inho¹,Chang Jonathan¹,Chang Michael T¹,Chang Patrick W¹,Chang Stephen¹,Chari Neel¹,Chassiakos Alexander J¹,Chen Iris E¹,Chen Vivian K¹,Chen Zheying¹,Cheng Marsha R¹,Chiang Mimi¹,Chiu Vivian¹,Choi Sharon¹,Chung Jun Ho¹,Contreras Liset¹,Corona Edgar¹,Cruz Courtney J¹,Cruz Renae L¹,Dang Jefferson M¹,Dasari Suhas P¹,De La Fuente Justin R O¹,Del Rio Oscar M A¹,Dennis Emily R¹,Dertsakyan Petros S¹,Dey Ipsita¹,Distler Rachel S¹,Dong Zhiqiao¹,Dorman Leah C¹,Douglass Mark A¹,Ehresman Allysen B¹,Fu Ivy H¹,Fua Andrea¹,Full Sean M¹,Ghaffari-Rafi Arash¹,Ghani Asmar Abdul¹,Giap Bosco¹,Gill Sonia¹,Gill Zafar S¹,Gills Nicholas J¹,Godavarthi Sindhuja¹,Golnazarian Talin¹,Goyal Raghav¹,Gray Ricardo¹,Grunfeld Alexander M¹,Gu Kelly M¹,Gutierrez Natalia C¹,Ha An N¹,Hamid Iman¹,Hanson Ashley¹,Hao Celesti¹,He Chongbin¹,He Mengshi¹,Hedtke Joshua P¹,Hernandez Ysrael K¹,Hlaing Hnin¹,Hobby Faith A¹,Hoi Karen¹,Hope Ashley C¹,Hosseinian Sahra M¹,Hsu Alice¹,Hsueh Jennifer¹,Hu Eileen¹,Hu Spencer S¹,Huang Stephanie¹,Huang Wilson¹,Huynh Melanie¹,Javier Carmen¹,Jeon Na Eun¹,Ji Sunjong¹,Johal Jasmin¹,John Amala¹,Johnson Lauren¹,Kadakia Saurin¹,Kakade Namrata¹,Kamel Sarah¹,Kaur Ravinder¹,Khatra Jagteshwar S¹,Kho Jeffrey A¹,Kim Caleb¹,Kim Emily Jin-Kyung¹,Kim Hee Jong¹,Kim Hyun Wook¹,Kim Jin Hee¹,Kim Seong Ah¹,Kim Woo Kyeom¹,Kit Brian¹,La Cindy¹,Lai Jonathan¹,Lam Vivian¹,Le Nguyen Khoi¹,Lee Chi Ju¹,Lee Dana¹,Lee Dong Yeon¹,Lee James¹,Lee Jason¹,Lee Jessica¹,Lee Ju-Yeon¹,Lee Sharon¹,Lee Terrence C¹,Lee Victoria¹,Li Amber J¹,Li Jialing¹,Libro Alexandra M¹,Lien Irvin C¹,Lim Mia¹,Lin Jeffrey M¹,Liu Connie Y¹,Liu Steven C¹,Louie Irene¹,Lu Shijia W¹,Luo William Y¹,Luu Tiffany¹,Madrigal Josef T¹,Mai Yishan¹,Miya Darron I¹,Mohammadi Mina¹,Mohanta Sayonika¹,Mokwena Tebogo¹,Montoya Tonatiuh¹,Mould Dallas L¹,Murata Mark R¹,Muthaiya Janani¹,Naicker Seethim¹,Neebe Mallory R¹,Ngo Amy¹,Ngo Duy Q¹,Ngo Jamie A¹,Nguyen Anh T¹,Nguyen Huy C X¹,Nguyen Rina H¹,Nguyen Thao T T¹,Nguyen Vincent T¹,Nishida Kevin¹,Oh Seo-Kyung¹,Omi Kristen M¹,Onglatco Mary C¹,Almazan Guadalupe Ortega¹,Paguntalan Jahzeel¹,Panchal Maharshi¹,Pang Stephanie¹,Parikh Harin B¹,Patel Purvi D¹,Patel Trisha H¹,Petersen Julia E¹,Pham Steven¹,Phan-Everson Tien M,Pokhriyal Megha¹,Popovich Davis W¹,Quaal Adam T¹,Querubin Karl¹,Resendiz Anabel¹,Riabkova Nadezhda¹,Rong Fred¹,Salarkia Sarah¹,Sama Nateli¹,Sang Elaine¹,Sanville David A¹,Schoen Emily R¹,Shen Zhouyang¹,Siangchin Ken¹,Sibal Gabrielle¹,Sin Garuem¹,Sjarif Jasmine¹,Smith Christopher J¹,Soeboer Annisa N¹,Sosa Cristian¹,Spitters Derek¹,Stender Bryan¹,Su Chloe C¹,Summapund Jenny¹,Sun Beatrice J¹,Sutanto Christine¹,Tan Jaime S¹,Tan Nguon L¹,Tangmatitam Parich¹,Trac Cindy K¹,Tran Conny¹,Tran Daniel¹,Tran Duy¹,Tran Vina¹,Truong Patrick A¹,Tsai Brandon L¹,Tsai Pei-Hua¹,Tsui C Kimberly¹,Uriu Jackson K¹,Venkatesh Sanan¹,Vo Maique¹,Vo Nhat-Thi¹,Vo Phuong¹,Voros Timothy C¹,Wan Yuan¹,Wang Eric¹,Wang Jeffrey¹,Wang Michael K¹,Wang Yuxuan¹,Wei Siman¹,Wilson Matthew N¹,Wong Daniel¹,Wu Elliott¹,Xing Hanning¹,Xu Jason P¹,Yaftaly Sahar¹,Yan Kimberly¹,Yang Evan¹,Yang Rebecca¹,Yao Tony¹,Yeo Patricia¹,Yip Vivian¹,Yogi Puja¹,Young Gloria Chin¹,Yung Maggie M¹,Zai Alexander¹,Zhang Christine¹,Zhang Xiao X¹,Zhao Zijun¹,Zhou Raymond¹,Zhou Ziqi¹,Abutouk Mona¹,Aguirre Brian¹,Ao Chon¹,Baranoff Alexis¹,Beniwal Angad¹,Cai Zijie¹,Chan Ryan¹,Chien Kenneth Chang¹,Chaudhary Umar¹,Chin Patrick¹,Chowdhury Praptee¹,Dalie Jamlah¹,Du Eric Y¹,Estrada Alec¹,Feng Erwin¹,Ghaly Monica¹,Graf Rose¹,Hernandez Eduardo¹,Herrera Kevin¹,Ho Vivien W¹,Honeychurch Kaitlyn¹,Hou Yurianna¹,Huang Jo M¹,Ishii Momoko¹,James Nicholas¹,Jang Gah-Eun¹,Jin Daphne¹,Juarez Jesse¹,Kesaf Ayse Elif¹,Khalsa Sat Kartar¹,Kim Hannah¹,Kovsky Jenna¹,Kuang Chak Lon¹,Kumar Shraddha¹,Lam Gloria¹,Lee Ceejay¹,Lee Grace¹,Li Li¹,Lin Joshua¹,Liu Josephine¹,Ly Janice¹,Ma Austin¹,Markovic Hannah¹,Medina Cristian¹,Mungcal Jonelle¹,Naranbaatar Bilguudei¹,Patel Kayla¹,Petersen Lauren¹,Phan Amanda¹,Phung Malcolm¹,Priasti Nadiyah¹,Ruano Nancy¹,Salim Tanveer¹,Schnell Kristen¹,Shah Paras¹,Shen Jinhua¹,Stutzman Nathan¹,Sukhina Alisa¹,Tian Rayna¹,Vega-Loza Andrea¹,Wang Joyce¹,Wang Jun¹,Watanabe Rina¹,Wei Brandon¹,Xie Lillian¹,Ye Jessica¹,Zhao Jeffrey¹,Zimmerman Jill¹,Bracken Colton¹,Capili Jason¹,Char Andrew¹,Chen Michel¹,Huang Pingdi¹,Ji Sena¹,Kim Emily¹,Kim Kenneth¹,Ko Julie¹,Laput Sean Louise G¹,Law Sam¹,Lee Sang Kuk¹,Lee Olivia¹,Lim David¹,Lin Eric¹,Marik Kyle¹,Mytych Josh¹,O'Laughlin Andie¹,Pak Jensen¹,Park Claire¹,Ryu Ruth¹,Shinde Ashwin¹,Sosa Manny¹,Waite Nick¹,Williams Mane¹,Wong Richard¹,Woo Jocelyn¹,Woo Jonathan¹,Yepuri Vishaal¹,Yim Dorothy¹,Huynh Dan¹,Wijiewarnasurya Dinali¹,Shapiro Casey³,Levis-Fitzgerald Marc³,Jaworski Leslie⁴,Lopatto David⁴,Clark Ira E¹²,Johnson Tracy¹²,Banerjee Utpal¹²⁵⁶

Affiliation:

1. Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA

2. Biomedical Research Minor, University of California, Los Angeles, Los Angeles, CA 90095, USA

3. Center for the Advancement of Teaching, University of California, Los Angeles, Los Angeles, CA 90095, USA

4. Department of Psychology, Grinnell College, Grinnell, IA 50112, USA

5. Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA

6. UCLA Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA 90095, USA

Abstract

Abstract Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach.

Funder

Howard Hughes Medical Institute (HHMI) through the Precollege and Undergraduate Science Education Program

UCLA Clinical and Translational Science Institute (CTSI; NIH National Center for Advancing Translational Science

HHMI Professors

Publisher

Oxford University Press (OUP)

Subject

Genetics (clinical),Genetics,Molecular Biology

Link

http://academic.oup.com/g3journal/article-pdf/11/1/1/36546396/jkaa028.pdf

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