Whole Animal Feeding FLat (WAFFL): a complete and comprehensive validation of a novel, high-throughput fly experimentation system

Author:

Jaime Maria D L A12,Salem Ghadi H3,Martinez Daniel J1,Karott Sean2,Flores Alejandra24,Palmer Cameron D2,Mahadevaraju Sharvani25,Krynitsky Jonathan3,Garmendia-Cedillos Marcial3,Anderson Sarah3,Harbison Susan6ORCID,Pohida Thomas J3,Ludington William B17ORCID,Oliver Brian25ORCID

Affiliation:

1. Department of Embryology, Carnegie Institution of Washington , Baltimore, MD 21218 , USA

2. Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , 50 South Drive, Bethesda, MD 20814 , USA

3. Instrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health , 12 South Dr, Bethesda, MD 20892 , USA

4. Department of Physiology and Biophysics, Case Western Reserve University , 10900 Euclid Ave, Cleveland, OH 44106 , USA

5. Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , 50 South Drive, Bethesda, MD 20814 , USA

6. Laboratory of Systems Genetics, National Heart Lung and Blood Institute, National Institutes of Health , 10 Center Drive, Bethesda, MD 20814 , USA

7. Department of Biology, Johns Hopkins University , Baltimore, MD 21218 , USA

Abstract

AbstractNon-mammalian model organisms have been essential for our understanding of the mechanisms that control development, disease, and physiology, but they are underutilized in pharmacological and toxicological phenotypic screening assays due to their low throughput in comparison with cell-based screens. To increase the utility of using Drosophila melanogaster in screening, we designed the Whole Animal Feeding FLat (WAFFL), a novel, flexible, and complete system for feeding, monitoring, and assaying flies in a high-throughput format. Our 3D printed system is compatible with inexpensive and readily available, commercial 96-well plate consumables and equipment. Experimenters can change the diet at will during the experiment and video record for behavior analysis, enabling precise dosing, measurement of feeding, and analysis of behavior in a 96-well plate format.

Funder

National Institute of Diabetes and Digestive and Kidney Diseases

National Institute of Biomedical Imaging and Bioengineering

National Heart Lung and Blood Institute

NSF

NIH

Publisher

Oxford University Press (OUP)

Subject

Genetics (clinical),Genetics,Molecular Biology

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