The chromosome-scale genome and the genetic resistance machinery against insect herbivores of the Mexican toloache, Datura stramonium

Author:

De-la-Cruz Ivan M12,Oyama Ken3,Núñez-Farfán Juan1ORCID

Affiliation:

1. Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México , Mexico City 04510 , Mexico

2. Department of Plant Protection Biology, Swedish University of Agricultural Sciences , Lomma, Alnarp 230 53 , Sweden

3. Escuela Nacional de Estudios Superiores (ENES), Universidad Nacional Autónoma de México (UNAM), Campus Morelia , Morelia, Michoacán 8701 , Mexico

Abstract

Abstract Plant resistance refers to the heritable ability of plants to reduce damage caused by natural enemies, such as herbivores and pathogens, either through constitutive or induced traits like chemical compounds or trichomes. However, the genetic architecture—the number and genome location of genes that affect plant defense and the magnitude of their effects—of plant resistance to arthropod herbivores in natural populations remains poorly understood. In this study, we aimed to unveil the genetic architecture of plant resistance to insect herbivores in the annual herb Datura stramonium (Solanaceae) through quantitative trait loci mapping. We achieved this by assembling the species’ genome and constructing a linkage map using an F2 progeny transplanted into natural habitats. Furthermore, we conducted differential gene expression analysis between undamaged and damaged plants caused by the primary folivore, Lema daturaphila larvae. Our genome assembly resulted in 6,109 scaffolds distributed across 12 haploid chromosomes. A single quantitative trait loci region on chromosome 3 was associated with plant resistance, spanning 0 to 5.17 cM. The explained variance by the quantitative trait loci was 8.44%. Our findings imply that the resistance mechanisms of D. stramonium are shaped by the complex interplay of multiple genes with minor effects. Protein–protein interaction networks involving genes within the quantitative trait loci region and overexpressed genes uncovered the key role of receptor-like cytoplasmic kinases in signaling and regulating tropane alkaloids and terpenoids, which serve as powerful chemical defenses against D. stramonium herbivores. The data generated in our study constitute important resources for delving into the evolution and ecology of secondary compounds mediating plant–insect interactions.

Funder

CONACyT

Fronteras de la Ciencia

CONACyT Mexico

PAPIIT-DGAPA

Publisher

Oxford University Press (OUP)

Subject

Genetics (clinical),Genetics,Molecular Biology

Reference104 articles.

1. Plant receptor-like serine threonine kinases: roles in signaling and plant defense;Afzal;Mol Plant Microbe Interact,2008

2. Plant defense syndromes;Agrawal;Ecology,2006

3. Gene ontology: tool for the unification of biology;Ashburner;Nat Genet,2000

4. Controlling the false discovery rate: a practical and powerful approach to multiple testing;Benjamini;J R Stat Soc Series B Stat Methodol,1995

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3