The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health-Reference-Cited by-同舟云学术

The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health

Published:2021-03-02 Issue:4 Volume:11 Page:
ISSN:2160-1836
Container-title:G3 Genes|Genomes|Genetics
language:en
Short-container-title:

Author:

Brose Julia¹,Lau Kin H¹,Dang Thu Thuy Thi²,Hamilton John P¹^ORCID,Martins Lívia do Vale¹³,Hamberger Britta⁴,Hamberger Bjoern⁴,Jiang Jiming¹⁵⁶^ORCID,O’Connor Sarah E⁷,Buell C Robin¹⁶⁸^ORCID

Affiliation:

1. Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA

2. Department of Chemistry, University of British Columbia Okanagan, Kelowna, BC V1V 1V7, Canada

3. Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE 50670-901, Brazil

4. Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI 48824, USA

5. Department of Horticulture, Michigan State University, East Lansing, MI 48824, USA

6. MSU AgBioResearch, Michigan State University, East Lansing, MI 48824, USA

7. Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, D-07745 Jena, Germany

8. Plant Resilience Institute, Michigan State University, East Lansing, MI 48824, USA

Abstract

Abstract Mitragyna speciosa (kratom) produces numerous compounds with pharmaceutical properties including the production of bioactive monoterpene indole and oxindole alkaloids. Using a linked-read approach, a 1,122,519,462 bp draft assembly of M. speciosa “Rifat” was generated with an N50 scaffold size of 1,020,971 bp and an N50 contig size of 70,448 bp that encodes 55,746 genes. Chromosome counting revealed that “Rifat” is a tetraploid with a base chromosome number of 11, which was further corroborated by orthology and syntenic analysis of the genome. Analysis of genes and clusters involved in specialized metabolism revealed genes putatively involved in alkaloid biosynthesis. Access to the genome of M. speciosa will facilitate an improved understanding of alkaloid biosynthesis and accelerate the production of bioactive alkaloids in heterologous hosts.

Funder

Michigan State University Strategic Partnership grant program

Michigan State University Distinguished Fellowship program (JB), European Research Commission

Natural Science and Engineering Research Council of Canada Discovery Grant

Department of Biochemistry and Molecular Biology

Michigan State University AgBioResearch

Publisher

Oxford University Press (OUP)

Subject

Genetics(clinical),Genetics,Molecular Biology

Link

http://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkab058/36941242/jkab058.pdf