A Short Historical Review of Water-Soluble Aminoplastic Resin Use for Lipid Ultrastructure Research

Abstract

Abstract In 1982, a water-soluble aminoplastic resin embedding method for electron microscopy (EM) was developed to study lipids. It involves a silylation technique for processing cured resins by converting them from hydrophilic to hydrophobic status, facilitating ultra-thin sectioning. The technique offers better preservation of lipovitellin material in frog (Rana amurensis) yolk platelets, and it shows membrane ultrastructure better than traditional epoxy embedding methods. An early (1982) pseudo-colored TEM image processing technique applied to the micrographs obtained by EM of silylated aminoplastic thin sections revealed the lipovitellin molecules, in relation to the biosynthesis of new membranes in the first cleavage of the egg, with remarkable clarity and explains the lipovitellin molecules in relation to the biosynthesis of new membranes. Because the original article was written in Chinese and published in China before its open policy, many scientists may not be aware of the publication. Thus, in this short review, the essential components of the procedure are presented in English to help guide investigators who might be interested in the use of water-soluble embedding methods for lipid research.