New Confocal Microscope for Quantitative Fluorescence Lifetime Imaging with Improved Reproducibility

Abstract

AbstractQuantitative single-molecule and time-resolved fluorescence techniques offer new insights into many samples in life and materials sciences. So far, their adoption has been slow because expert knowledge is required for correct data acquisition and analysis. Now, PicoQuant has developed a single-photon counting confocal microscope, called Luminosa. It combines state-of-the-art hardware with cutting-edge software to deliver high-quality data while simplifying daily operation. The system software includes context-based workflows for each technique, which improve accuracy, reproducibility, and quality of the data. This article showcases how this is achieved for fluorescence lifetime imaging (FLIM).