Artificial-regulated synthesis of nanocrystals in live cells

Abstract

ABSTRACT Live cells, as reservoirs of biochemical reactions, can serve as amazing integrated chemical plants where precursor formation, nucleation and growth of nanocrystals, and functional assembly can be carried out accurately following an artificial program. It is crucial but challenging to deliberately direct intracellular pathways to synthesize desired nanocrystals that cannot be produced naturally in cells, because the relevant reactions exist in different spatiotemporal dimensions and will never encounter spontaneously. This article summarizes progress in the introduction of inorganic functional nanocrystals into live cells via the ‘artificial-regulated space–time-coupled live-cell synthesis’ strategy. We also describe ingenious bio-applications of the nanocrystal–cell systems, and quasi-biosynthesis strategies expanded from live-cell synthesis. Artificial-regulated live-cell synthesis—which involves the interdisciplinary application of biology, chemistry, nanoscience and medicine—will enable researchers to better exploit the unanticipated potentialities of live cells and open up new directions in synthetic biology.