Seasonal dynamics of cell-to-cell transport in angiosperm wood

Author:

Słupianek Aleksandra1ORCID,Myśkow Elżbieta1ORCID,Kasprowicz-Maluśki Anna2ORCID,Dolzblasz Alicja1ORCID,Żytkowiak Roma3ORCID,Turzańska Magdalena1ORCID,Sokołowska Katarzyna1ORCID

Affiliation:

1. Department of Plant Developmental Biology, Faculty of Biological Sciences, University of Wrocław , Kanonia 6/8, 50-328 Wrocław , Poland

2. Department of Molecular and Cellular Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University , Uniwersytetu Poznańskiego 6, Poznań 61-614 , Poland

3. Institute of Dendrology, Polish Academy of Sciences , Parkowa 5, 62-035 Kórnik , Poland

Abstract

Abstract This study describes the seasonal changes in cell-to-cell transport in three selected angiosperm tree species, Acer pseudoplatanus (maple), Fraxinus excelsior (ash), and Populus tremula × tremuloides (poplar), with an emphasis on the living wood component, xylem parenchyma cells (XPCs). We performed anatomical studies, dye loading through the vascular system, measurements of non-structural carbohydrate content, immunocytochemistry, inhibitory assays and quantitative real-time PCR to analyse the transport mechanisms and seasonal variations in wood. The abundance of membrane dye in wood varied seasonally along with seasonally changing tree phenology, cambial activity, and non-structural carbohydrate content. Moreover, dyes internalized in vessel-associated cells and ‘trapped’ in the endomembrane system are transported farther between other XPCs via plasmodesmata. Finally, various transport mechanisms based on clathrin-mediated and clathrin-independent endocytosis, and membrane transporters, operate in wood, and their involvement is species and/or season dependent. Our study highlights the importance of XPCs in seasonally changing cell-to-cell transport in both ring-porous (ash) and diffuse-porous (maple, poplar) tree species, and demonstrates the involvement of both endocytosis and plasmodesmata in intercellular communication in angiosperm wood.

Funder

National Science Centre, Poland

Ministry of Education and Science, Poland

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

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