Gene editing of non-coding regulatory DNA and its application in crop improvement

Author:

Chen Yu-Hao1ORCID,Lu Jun1,Yang Xia1,Huang Li-Chun1,Zhang Chang-Quan1,Liu Qiao-Quan12ORCID,Li Qian-Feng12ORCID

Affiliation:

1. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University , Yangzhou 225009, Jiangsu , China

2. Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province, Yangzhou University , Yangzhou 225009, Jiangsu , China

Abstract

Abstract The development of the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) system has provided precise and efficient strategies to edit target genes and generate transgene-free crops. Significant progress has been made in the editing of protein-coding genes; however, studies on the editing of non-coding DNA with regulatory roles lags far behind. Non-coding regulatory DNAs, including those which can be transcribed into long non-coding RNAs (lncRNAs), and miRNAs, together with cis-regulatory elements (CREs), play crucial roles in regulating plant growth and development. Therefore, the combination of CRISPR/Cas technology and non-coding regulatory DNA has great potential to generate novel alleles that affect various agronomic traits of crops, thus providing valuable genetic resources for crop breeding. Herein, we review recent advances in the roles of non-coding regulatory DNA, attempts to edit non-coding regulatory DNA for crop improvement, and potential application of novel editing tools in modulating non-coding regulatory DNA. Finally, the existing problems, possible solutions, and future applications of gene editing of non-coding regulatory DNA in modern crop breeding practice are also discussed.

Funder

Ministry of Science and Technology of China

National Natural Science Foundation of China

Programs from Government of Jiangsu Province

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

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