Multiomics comparison among populations of three plant sources of Amomi Fructus
Author:
Chen Xinlian12, Sun Shichao2, Han Xiaoxu2, Li Cheng2, Wang Fengjiao2, Nie Bao2, Hou Zhuangwei2, Yang Song2, Ji Jiaojiao2, Li Ge3, Wang Yanqian3, Han Xiaoyu12, Yue Jianjun14, Li Cui5, Li Wei2, Zhang Lixia3, Yang Depo1, Wang Li26
Affiliation:
1. Sun Yat-Sen University School of Pharmaceutical Sciences, , 510006 Guangzhou, China 2. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, , 518120 Shenzhen, China 3. Chinese Academy of Medical Sciences Yunnan Key Laboratory of Southern Medicine Utilization, Yunnan Branch Institute of Medicinal Plant Development, , 666100 Jinghong, China 4. West Yunnan University of Applied Sciences School of Traditional Dai-Thai Medicine, , 666100 Jinghong, China 5. Guangxi Botanical Garden of Medicinal Plants National Center for TCM Inheritance and Innovation, , 530023 Nanning, China 6. Chinese Academy of Agricultural Sciences Kunpeng Institute of Modern Agriculture at Foshan, , 528200 Foshan, China
Abstract
ABSTRACT
Amomi Fructus (Sharen, AF) is a traditional Chinese medicine (TCM) from three source species (or varieties), including Wurfbainia villosa var. villosa (WVV), W. villosa var. xanthioides (WVX), or W. longiligularis (WL). Among them, WVV has been transplanted from its top-geoherb region, Guangdong, to its current main production area, Yunnan, for >50 years in China. However, the genetic and transcriptomic differentiation among multiple AF source species (or varieties) and between the origin and transplanted populations of WVV is unknown. In our study, the observed overall higher expression of terpenoid biosynthesis genes in WVV than in WVX provided possible evidence for the better pharmacological effect of WVV. We also screened six candidate borneol dehydrogenases (BDHs) that potentially catalyzed borneol into camphor in WVV and functionally verified them. Highly expressed genes at the P2 stage of WVV, Wv05G1424 and Wv05G1438, were capable of catalyzing the formation of camphor from (+)-borneol, (−)-borneol and DL-isoborneol. Moreover, the BDH genes may experience independent evolution after acquiring the ancestral copies, and the following tandem duplications might account for the abundant camphor content in WVV. Furthermore, four populations of WVV, WVX, and WL are genetically differentiated, and the gene flow from WVX to WVV in Yunnan contributed to the greater genetic diversity in the introduced population (WVV-JH) than in its top-geoherb region (WVV-YC), which showed the lowest genetic diversity and might undergo genetic degradation. In addition, terpene synthesis (TPS) and BDH genes were selected among populations of multiple AF source species (or varieties) and between the top- and non-top-geoherb regions, which might explain the difference in metabolites between these populations. Our findings provide important guidance for the conservation, genetic improvement, and industrial development of the three source species (or varieties) and for identifying top-geoherbalism with molecular markers, and proper clinical application of AF.
Publisher
Oxford University Press (OUP)
Subject
Horticulture,Plant Science,Genetics,Biochemistry,Biotechnology
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