Liquid Chromatographic Determination of Pyrethroids as Active Ingredients in Pesticide Formulations

Abstract

Abstract A normal-phase liquid chromatographic method for determining pyrethroids as active ingredients in pesticide formulations was developed. The method uses isooctane–ethyl acetate as mobile phase and UV detection at 275 nm. The method was evaluated for the following 7 pyrethroid active ingredients: α-cypermethrin, cypermethrin, deltamethrin, esfenvalerate, fenvalerate, fenpropathrin, and permethrin. Evaluation covered 3 formulation types (emulsifiable concentrate, suspension concentrate, and water-dispersible granule) containing a single pyrethroid active ingredient. The method separates diastereomers of cypermethrin, fenvalerate, and permethrin and can be used to determine total content as well as relative distribution of diastereomers.