Chloramphenicol Extraction from Milk by Using the Diphasic Dialysis Method Followed by Liquid Chromatographic Determination

Author:

Bayo Javier1,Moreno Miguel A1,Prieta Javier1,Díaz Susana1,Suárez Guillermo1,Domínguez Lucas1

Affiliation:

1. Universidad Complutense, Facultad de Veterinaria, Departamento PatologIa Animal I, 28040 Madrid, Spain

Abstract

Abstract An economical, fast, and simple method for the extraction and liquid chromatographic determination of chloramphenicol (CAP) in milk is described. CAP is extracted by using a recently developed membrane-based method named “diphasic dialysis.” CAP is detected and quantitated in the organic solvent used in dialysis without additional cleanup steps by reversed-phase liquid chromatography and UV detection (270 nm). The determination imit of CAP in milk was about 5 μg/L, although as little as 1 μg/L could be detected under optimal working conditions.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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