Moslosooflavone protects against brain injury induced by hypobaric hypoxic via suppressing oxidative stress, neuroinflammation, energy metabolism disorder, and apoptosis
Author:
Zhang Jie12,
Zhao Tong3,
Zhang Pengpeng2,
Shi Zhiqun2,
Da Qingyue12,
Ma Huiping2,
Jing Linlin12ORCID
Affiliation:
1. Department of Pharmacy, The First Affiliated Hospital of Xi’an Jiaotong University , Xi’an, Shaanxi 710061 , China
2. Department of Pharmacy, The 940th Hospital of Joint Logistics Support Force of PLA , Lanzhou, Gansu 730050 , China
3. Department of Pharmacy, Gansu Provincial Maternity and Child Care Hospital , Lanzhou, Gansu 730050 , China
Abstract
Abstract
Objectives
To investigate the protect effect of moslosooflavone against brain injury induced by hypobaric hypoxia (HH) in mice.
Methods
Protective effects of moslosooflavone in oxidative stress, neuroinflammation, energy metabolism disorder, and apoptosis were studied in HH-induced brain damage mice. The pathological morphology in the cortex of mice was determined by hematoxylin and eosin staining. The related protein expressions were detected by western blot.
Key findings
Moslosooflavone improved HH-induced brain histopathological changes, reduced the contents of ROS and MDA, and elevated the levels of antioxidant enzymes and GSH in HH-exposed brains of mice. Moslosooflavone also markedly enhanced the ATPase activities and PK, ATP contents, while reducing LDH activity and the LD, TNF-α, IL-1β, and IL-6 contents HH-exposed brains of mice. In addition, moslosooflavone notably decreased the expression of HIF-1α, VEGF, Bax, and cleaved caspase-3 dramatically increasing the expression of Bcl-2, Nrf2, and HO‑1 in HH-exposed brains of mice.
Conclusions
Our current studies indicate that moslosooflavone protects HH-induced brain injury possibly through alleviating oxidative stress and neuroinflammation, maintaining the balance of energy metabolism, and inhibiting cell apoptosis.
Funder
National Natural Science Foundation of China
Military Logistic Support Capability Enhancement
Natural Science Foundation of Gansu Province
Institutional Foundation
Xi’an Jiaotong University
Publisher
Oxford University Press (OUP)
Subject
Pharmaceutical Science,Pharmacology
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