Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering

Author:

Rasila Tiina S12,Pulkkinen Elsi1,Kiljunen Saija1,Haapa-Paananen Saija1,Pajunen Maria I3,Salminen Anu4,Paulin Lars2,Vihinen Mauno5,Rice Phoebe A6ORCID,Savilahti Harri12

Affiliation:

1. Division of Genetics and Physiology, Department of Biology, FI-20014 University of Turku, Turku, Finland

2. Institute of Biotechnology, Viikki Biocenter, P. O. Box 56, FI-00014 University of Helsinki, Helsinki, Finland

3. Division of Biochemistry and Biotechnology, Department of Biosciences, FI-00014 University of Helsinki, Helsinki, Finland

4. Department of Biochemistry, FI-20014 University of Turku, Turku, Finland

5. Department of Experimental Medical Science, Lund University, SE-221 84, Lund, Sweden

6. Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA

Funder

Academy of Finland

Finnish National Technology Agency TEKES

Publisher

Oxford University Press (OUP)

Subject

Genetics

Reference50 articles.

1. Mobile DNA III

2. DNA transposition at work;Hickman;Chem. Rev.,2016

3. The expanding universe of transposon technologies for gene and cell engineering;Ivics;Mob. DNA,2010

4. Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery;Kiljunen;BMC Biol.,2014

5. Random mutagenesis of the hyperthermophilic archaeon Pyrococcus furiosus using in vitro mariner transposition and natural transformation;Guschinskaya;Sci. Rep.,2016

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