Validation of Three Rapid Screening Methods for Detection of Verotoxin-Producing Escherichia coli in Foods: Interlaboratory Study
Author:
Capps Katherine L1, McLaughlin Emiline M1, Murray Alistair W A1, Aldus Clare F2, Wyatt Gary M2, Peck Michael W2, Van Amerongen Aart3, Ariëns Renata M C3, Wichers Jan H3, Baylis Christopher L4, Wareing David R A5, Bolton Frederick J5, Aird H, Allen R, Anderson P, Boughtflower M, Chen Q, Davies A, Dennis J, Gibson S J, Green R A, Hilton J, Jorgensen F, Leuschner R, Loder C, Mackey B, Meldrum R, Millar I, Reid T, Robinson A J, Robinson; I, Smith H, Surman S, Vickers D, Wood M,
Affiliation:
1. Central Science Laboratory, Sand Hutton, York, North Yorkshire, YO41 1LZ, United Kingdom 2. Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, United Kingdom 3. Agrotechnology and Food Innovations A&F B.V., Bornsesteeg 59, 6708 PD Wageningen, The Netherlands 4. Campden & Chorleywood Food Research Association, Chipping Campden, Gloucestershire, GL55 6LD, United Kingdom 5. Preston Public Health Laboratory, Royal Preston Hospital, PO Box 202, Sharoe Green LN, Fulwood, Preston, Lancashire, PR2 9HG, United Kingdom
Abstract
Abstract
An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (<9 to 375 cells/25 g) of 6 test strains of E. coli (serogroups O26, O103, O111, O145, and O157) with differing VT-producing capabilities. Five replicates for each test strain and 5 uninoculated samples were prepared for each food type. Fourteen participating laboratories analyzed samples using the LFIA, 9 analyzed the samples by ELISA, and 9 by PCR. The LFIA for O157 and VT had a specificity (correct identification of negative samples) of 92 and 94%, respectively, and a sensitivity (correct identification of positive samples) of 94 and 55%, respectively. The VT-ELISA and VT-PCR
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
17 articles.
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