microRNA Deficiency in VIP+ Interneurons Leads to Cortical Circuit Dysfunction-Reference-Cited by-同舟云学术

microRNA Deficiency in VIP+ Interneurons Leads to Cortical Circuit Dysfunction

Published:2019-11-04 Issue:4 Volume:30 Page:2229-2249
ISSN:1047-3211
Container-title:Cerebral Cortex
language:en
Short-container-title:

Author:

Qiu Fang¹,Mao Xingfeng²,Liu Penglai²,Wu Jinyun¹,Zhang Yuan³,Sun Daijing³,Zhu Yueyan³,Gong Ling¹,Shao Mengmeng⁴,Fan Keyang¹,Chen Junjie¹,Lu Jiangteng⁴,Jiang Yan³,Zhang Yubin⁵,Curia Giulia⁶⁷,Li Anan²,He Miao¹

Affiliation:

1. Department of Neurology, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institutes of Brain Science, Zhongshan Hospital, Fudan University, Shanghai 200032, China

2. Jiangsu Key Laboratory of Brain Disease and Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, Xuzhou 221004, China

3. State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China

4. Department of Anatomy and Physiology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China

5. Department of Toxicology, School of Public Health, Fudan University, Shanghai 200032, China

6. Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41121, Italy

7. Center for Neuroscience and Neurotechnology, University of Modena and Reggio Emilia, Modena 41121, Italy

Abstract

Abstract Genetically distinct GABAergic interneuron subtypes play diverse roles in cortical circuits. Previous studies revealed that microRNAs (miRNAs) are differentially expressed in cortical interneuron subtypes, and are essential for the normal migration, maturation, and survival of medial ganglionic eminence-derived interneuron subtypes. How miRNAs function in vasoactive intestinal peptide expressing (VIP+) interneurons derived from the caudal ganglionic eminence remains elusive. Here, we conditionally removed Dicer in postmitotic VIP+ interneurons to block miRNA biogenesis. We found that the intrinsic and synaptic properties of VIP+ interneurons and pyramidal neurons were concordantly affected prior to a progressive loss of VIP+ interneurons. In vivo recording further revealed elevated cortical local field potential power. Mutant mice had a shorter life span but exhibited better spatial working memory and motor coordination. Our results demonstrate that miRNAs are indispensable for the function and survival of VIP+ interneurons, and highlight a key role of VIP+ interneurons in cortical circuits.

Funder

National Key R&D Program of China

National Natural Science Foundation of China

Shanghai Science and Technology Commission Innovation Fund

Shanghai Commission of Health Youth Program

Shanghai Rising-Star Program

Shanghai Municipal Science and Technology Major Project

Publisher

Oxford University Press (OUP)

Subject

Cellular and Molecular Neuroscience,Cognitive Neuroscience

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