A leaderless mRNA including tRNA-like sequence encodes a small peptide that regulates the expression of GcvB small RNA in <i>Escherichia coli</i>-Reference-Cited by-同舟云学术

A leaderless mRNA including tRNA-like sequence encodes a small peptide that regulates the expression of GcvB small RNA in Escherichia coli

Published:2022-01-27 Issue:4 Volume:171 Page:459-465
ISSN:0021-924X
Container-title:The Journal of Biochemistry
language:en
Short-container-title:

Author:

Muto Akira¹,Goto Simon¹,Kurita Daisuke¹,Ushida Chisato¹,Soma Akiko²,Himeno Hyota¹

Affiliation:

1. Department of Biochemistry and Molecular Biology, Faculty of Agriculture and Bioscience, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan

2. Graduate School of Horticulture, Chiba University, 648 Matsudo, Chiba 271-8510, Japan

Abstract

Abstract A tRNA-like sequence conserved in the genomes of all Escherichia coli strains was found. The sequence resembles arginine-tRNA, which is present in E. coli pathogenic islands and phages. Expression experiments revealed that this sequence is a part of a leaderless mRNA encoding a short peptide (60 amino acids: XtpA). A deletion mutant of this gene is more sensitive than wild-type cell to several aminoglycoside antibiotics at low concentrations. Further analyses indicated that XtpA positively regulates the expression of GcvB small RNA, which is involved in the intrinsic resistance to aminoblycosides in E. coli.

Publisher

Oxford University Press (OUP)

Subject

Molecular Biology,Biochemistry,General Medicine

Link

https://academic.oup.com/jb/advance-article-pdf/doi/10.1093/jb/mvac007/42578908/mvac007.pdf

Reference20 articles.

1. Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology;Katz;Mol. Microbiol.,2016

2. A bacterial RNA that functions as both a tRNA and an mRNA;Muto;Trends Biochem. Sci.,1998

3. The tmRNA system for translational surveillance and ribosome rescue;Moor;Ann. Rev. Biochem.,2007

4. tRNADB-CE. tRNA gene database curated manually by experts;Abe;Nucleic Acids Res.,2011

5. One-step inactivation of chromosomal genes in Escherichia coli K12 using PCR products;Datsenko;Proc. Natl. Acad. Sci. USA,2000

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. In silico investigations and molecular insights for designing tRNA-encoded peptides as potential therapeutics for targeting over-expressed receptors in breast cancer;Journal of Biomolecular Structure and Dynamics;2024-02-09

2. RegulonDB v12.0: a comprehensive resource of transcriptional regulation in E. coli K-12;Nucleic Acids Research;2023-11-16