Detection of Bovine Meat and Bone Meal in Animal Feed at a Level of 0.1%-Reference-Cited by-同舟云学术

Detection of Bovine Meat and Bone Meal in Animal Feed at a Level of 0.1%

Published:2006-11-01 Issue:6 Volume:89 Page:1443-1446
ISSN:1060-3271
Container-title:Journal of AOAC INTERNATIONAL
language:en
Short-container-title:

Author:

Aarts Henk J M¹,Bouw El M¹,Buntjer Jaap B²,Lenstra Johannes A³,Raamsdonk Leo W D van¹

Affiliation:

1. RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, The Netherlands

2. Keygene N.V., PO Box 216, 6700 AE Wageningen, The Netherlands

3. Utrecht University, Faculty of Veterinary Medicine, Yalelaan 8, 3584 CM Utrecht, The Netherlands

Abstract

Abstract For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR® Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133C for 20 min, a contamination level of 0.1% was detected.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

Link

http://academic.oup.com/jaoac/article-pdf/89/6/1443/32419034/jaoac1443.pdf

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