Reassembling green fluorescent protein for in vitro evaluation of trans-translation-Reference-Cited by-同舟云学术

Reassembling green fluorescent protein for in vitro evaluation of trans-translation

Published:2020-01-10 Issue:4 Volume:48 Page:e22-e22
ISSN:0305-1048
Container-title:Nucleic Acids Research
language:en
Short-container-title:

Author:

Guyomar Charlotte¹,Thépaut Marion¹²,Nonin-Lecomte Sylvie³,Méreau Agnès¹,Goude Renan¹,Gillet Reynald¹^ORCID

Affiliation:

1. Univ. Rennes, CNRS, Institut de Génétique et Développement de Rennes (IGDR) UMR6290, Rennes, France

2. SATT Ouest-Valorisation, Rennes, France

3. Université de Paris, CiTCoM, CNRS, UMR 8038, F-75006 Paris, France

Abstract

Abstract In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) in bacteria. This system is based on an engineered tmRNA variant that reassembles the green fluorescent protein (GFP) when trans-translation is active. Our system is adapted for high-throughput screening of chemical compounds by fluorescence.

Funder

Direction Générale de l’Armement

Joint Programming Initiative on Antimicrobial Resistance

SATT Ouest-Valorisation

CNRS

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

http://academic.oup.com/nar/article-pdf/48/4/e22/32615279/gkz1204.pdf

Reference33 articles.