Wolbachia (Rickettsiales: Alphaproteobacteria) Infection in the Leafhopper Vector of Sugarcane White Leaf Disease

Author:

Wangkeeree Jureemart1,Tewaruxsa Panida1,Roddee Jariya2,Hanboonsong Yupa3

Affiliation:

1. Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University Rangsit Centre, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand

2. School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Suranaree, Muang, Nakhon Ratchasima, Thailand

3. Department of Entomology, Faculty of Agriculture, Khon Kaen University, Nai Muang, Muang, Khon Kaen, Thailand

Abstract

Abstract Wolbachia is a maternally inherited bacterium ubiquitous in insects that has attracted interest as a prospective insect pest-control agent. Here, we detected and characterized Wolbachia in the leafhoppers Matsumuratettix hiroglyphicus (Matsumura) (Cicadellidae: Hemiptera) and Yamatotettix flavovittatus Matsumura (Cicadellidae: Hemiptera), insect vectors of the phytoplasma that cause white leaf disease in sugarcane. The 16S rRNA and wsp gene markers revealed that Wolbachia was not present in the M. hiroglyphicus but naturally occurs in Y. flavovittatus. Additionally, the infection rates in adult leafhoppers ranged from 0 to 100% depending on geographic location. Moreover, Wolbachia was detected in the eggs and first- to fifth-instar nymphs of Y. flavovittatus. A phylogenic tree of Wolbachia indicated that it resided in the monophyletic supergroup B clade and clustered in the Ori subgroup. Furthermore, fluorescence in situ hybridization revealed that Wolbachia localized to the egg apices, randomly distributed in the egg cytoplasm, and was concentrated in the nymph and adult bacteriomes, as well as occasional detection in the thorax and abdomen. To the best of our knowledge, the present study is the first to demonstrate the prevalence of Wolbachia in the leafhopper Y. flavovittatus. The obtained results would provide useful information for the future development of Wolbachia as a biological control agent for the leafhopper vectors.

Funder

Thammasat University

Thailand Research Fund

Publisher

Oxford University Press (OUP)

Subject

Insect Science,General Medicine

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