Affiliation:
1. Laboratorio de Entomología Universidad de la Amazonía, LEUA, Grupo de Investigación en Entomología Universidad de la Amazonia (GIEUA), Universidad de la Amazonia, Facultad de Ingeniería , Florencia, Caquetá , Colombia
2. Museo Entomológico UNAB, Sistemática de Insectos Agronomía, SIA, Facultad de Ciencias Agrarias, Universidad Nacional de Colombia , Bogotá , Colombia
Abstract
AbstractEthanol (EtOH) 70% is commonly used in collections to preserve and store many unprepared soft-bodied Chalcidoidea. Specimens air-dried directly from alcohol, however, often suffer from collapse of some body parts, making subsequent observation of their morphology difficult or even impossible. We propose an inexpensive method for processing and dry-mounting specimens of Chalcidoidea, using a chemical process including amyl acetate. Four treatments using amyl acetate at different concentrations and exposure times were evaluated for specimens of Eulophidae, Mymaridae, Encyrtidae, Aphelinidae, Pteromalidae, and Trichogrammatidae. Treatment with amyl acetate resulted in specimens of consistently higher quality. Based on our results, treatment of specimens for 1 h with 50:50 amyl acetate and ethanol mixture, followed by treatment for 1 h with 100% amyl acetate, yielded specimens adequate for morphological observations for most of the families. Further experiments are required, however, to optimize this approach for Trichogrammatidae and Eulophidae. This method is a relatively simple, inexpensive, and safe alternative to other methods commonly used for restoring Chalcidoidea preserved in alcohol.
Funder
Ministerio de Ciencia
Tecnología e Innovación MINCIENCIAS
General Royalty System
Publisher
Oxford University Press (OUP)
Subject
Insect Science,General Medicine
Cited by
3 articles.
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