Affiliation:
1. Research Institute of Resources Insect, Chinese Academy of Forestry, Kunming, China
2. NanJing Forestry University, Nanjing, China
3. The Key Laboratory of Cultivating and Utilization of Resources Insects, State Forestry Administration, Kunming, China
Abstract
Abstract
Insect hormones regulate metamorphosis including that leading to sexual dimorphism. Using RNA-Seq, we discovered that the second-instar male larva (SM) of the white wax insect, Ericerus pela, have 5,968 and 8,620 differentially expressed transcripts compared with the second-instar female larva (SF) and the first-instar male larva (FM), respectively. The expression levels of genes involved in the apoptosis of old tissues and the reconstruction of new ones in the SM significantly enhanced, while the SF mainly has enhanced expression levels of anabolic genes such as chitin. We predicted that the second-instar larvae are the developmental origin of sexual dimorphic metamorphosis. Meanwhile, in the juvenile hormone (JH) metabolic pathway, CYP15A1 and JH esterase (JHE) are differentially expressed; and in the 20-hydroxyecdysone (20E) metabolic pathway, CYP307A1, CYP314A1, and CYP18A1 are differentially expressed. In the SM, the expression levels of CYP307A1 and CYP314A1 are significantly increased, whereas the expression level of CYP18A1 is significantly decreased; in the SF, the expression levels of the above genes are opposite to that of the SM. Expression trends of RNA-seq is consistent with the expression level of qRT–PCR, and seven of them are highly correlated (R ≥ 0.610) and four are moderately correlated (0.588 ≥ R ≥ 0.542).
Funder
Introduction Project of International Advanced Technology of Forestry
National Natural Science Foundation of China
Fundamental Research Funds for Central Non-profit Research Institution
Publisher
Oxford University Press (OUP)
Subject
Insect Science,General Medicine
Cited by
10 articles.
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