Transcriptome analysis used to identify and characterize odorant binding proteins in <i>Agasicles hygrophila</i> (Coleoptera: Chrysomelidae)-Reference-Cited by-同舟云学术

Transcriptome analysis used to identify and characterize odorant binding proteins in Agasicles hygrophila (Coleoptera: Chrysomelidae)

Published:2023-09-01 Issue:5 Volume:23 Page:
ISSN:1536-2442
Container-title:Journal of Insect Science
language:en
Short-container-title:

Author:

Dong Changhong¹²^ORCID,Huang Cong²³^ORCID,Ning Xiaoyu²⁴,Liu Bo²,Qiao Xi²^ORCID,Qian Wanqiang²^ORCID,Zhu Daohong¹^ORCID,Wan Fanghao²³^ORCID

Affiliation:

1. Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Science and Technology, Central South University of Forestry and Technology (CSUFT) , Changsha , China

2. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences , Shenzhen , China

3. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , China

4. College of Plant Protection/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, Hainan University , Haikou , China

Abstract

Abstract The transcriptomes of Agasicles hygrophila eggs and first instar larvae were analyzed to explore the olfactory mechanism of larval behavior. The analysis resulted in 135,359 unigenes and the identification of 38 odorant-binding proteins (OBPs), including 23 Minus-C OBPs, 8 Plus-C OBPs, and 7 Classic OBPs. Further analysis of differentially expressed genes (DEGs) revealed 10 DEG OBPs, with 5 (AhygOBP5, AhygOBP9, AhygOBP12, AhygOBP15 and AhygOBP36) up-regulated in first instar larvae. Verification of expression patterns of these 5 AhygOBPs using qPCR showed that AhygOBP9 and AhygOBP36 were mainly expressed in the adult stage with gradually increasing expression in the larval stage. AhygOBP5, AhygOBP12, and AhygOBP15 were not expressed in eggs and pupae, and their expression in larvae and adults showed no clear pattern. These 5 AhygOBPs may play an olfactory role in larval behavior, providing a basis for further investigation of their specific functions and clarifying the olfactory mechanism of A. hygrophila.

Funder

National Key Research and Development Program of China

Shenzhen Science and Technology Program

Publisher

Oxford University Press (OUP)

Subject

Insect Science,General Medicine

Link

https://academic.oup.com/jinsectscience/article-pdf/23/5/16/53778717/iead081.pdf

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2. Correction to: Transcriptome analysis used to identify and characterize odorant binding proteins in Agasicles hygrophila (Coleoptera: Chrysomelidae);Journal of Insect Science;2023-11-01