A novel Bacillus sp. with antagonistic activity against a plant pathogen, Fusarium graminearum, and its potential antagonistic mechanism

Author:

Shen Shanrui1234,Yu Feifan1,Hao Xinyi1,Chen Jing1,Gao Huan1234,Lai Xiaofang1234

Affiliation:

1. Jiangsu Ocean University Jiangsu Key Laboratory of Marine Bioresources and Environment /Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province /School of Marine Science and Fisheries , Lianyungang 222005, Jiangsu , China

2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang 222005, Jiangsu , China

3. Marine Resource Development Institute of Jiangsu (Lianyungang) , Lianyungang 222005, Jiangsu , China

4. The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm , Nanjing 210014, Jiangsu , China

Abstract

Abstract Fusarium head blight (FHB) is a wheat disease caused by the plant pathogen Fusarium graminearum, which leads to crop yield losses and agricultural economic losses, as well as poses a threat to the environment and human health. Effective biocontrol of F. graminearum is urgent. An antagonistic strain HZ-5 with 59.2% antagonistic activity against F. graminearum in vitro had been isolated from sea mud of Haizhou Bay using a dual-culture assay, which was highly homologous with Bacillus halosaccharovorans according to the 16S rRNA sequence. The antagonistic activity of HZ-5 had been further studied. HZ-5 had a broad range of antagonistic activity against another six plant pathogenic fungi and was effective in controlling FHB of wheat in pot experiment. The substances with antagonistic activity were temperature insensitive, and had been purified by HPLC (High Performance Liquid Chromatography) to prove to be secreted lipopeptides. The antagonistic substances induced the biosynthesis of chitin and glycerol, while ergosterol , cholesterol, and phosphatidylcholine reduced their inhibitory effects on F. graminearum. These data would be helpful to provide a better biocontrol strain against FHB, and to provide important basis to elucidate the antagonistic mechanism of biocontrol.

Funder

PAPD

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology

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