Investigating the crucial role of selected Bifidobacterium probiotic strains in preventing or reducing inflammation by affecting the autophagy pathway

Author:

Torkamaneh Mahdi1,Torfeh Mahnaz1,Jouriani Fatemeh Haririzadeh1,Sepehr Amin1,Ashrafian Fatemeh2,Aghamohammad Shadi1,Rohani Mahdi1ORCID

Affiliation:

1. Department of Bacteriology, Pasteur Institute of Iran , Tehran 1316943551 , Iran

2. Clinical Research Department, Pasteur Institute of Iran , Tehran 1316943551 , Iran

Abstract

Abstract Several studies have shown that probiotics can prevent and reduce inflammation in inflammation-related diseases. However, few studies have focused on the interaction between host and probiotics in modulating the immune system through autophagy. Therefore, we aimed to investigate the preventive and/or therapeutic effects of native potential probiotic breast milk-isolated Bifidobacterium spp. (i.e. B. bifidum, B. longum, and B. infantis) on the inflammatory cascade by affecting autophagy gene expression 24 and 48 h after treatment. Autophagy genes involved in different stages of the autophagy process were selected by quantitative polymerase chain reaction (qPCR). Gene expression investigation was accomplished by exposing the human colorectal adenocarcinoma cell line (HT-29) to sonicated pathogens (1.5 × 108 bacterial CFU ml−1) and adding Bifidobacterium spp. (MOI10) before, after, and simultaneously with induction of inflammation. An equal volume of RPMI medium was used as a control. Generally, our native potential probiotic Bifidobacterium spp. can increase the autophagy gene expression in comparison with pathogen. Moreover, an increase in gene expression was observed with our probiotic strains’ consumption in all stages of autophagy. Totally, our selected Bifidobacterium spp. can increase autophagy gene expression before, simultaneously, and after the inflammation induction, so they can prevent and reduce inflammation in an in vitro model of inflammation.

Funder

Pasteur Institute of Iran

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology

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