Cytotoxicity assessment and antimicrobial effects of cell-free supernatants from probiotic lactic acid bacteria and yeast against multi-drug resistant Escherichia coli

Author:

Ozma Mahdi Asghari1ORCID,Ghotaslou Reza2,Asgharzadeh Mohammad3,Abbasi Amin4,Rezaee Mohammad Ahangarzadeh5,Kafil Hossein Samadi6

Affiliation:

1. Student Research Committee, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran

2. Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran

3. Biotechnology Research Center, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran

4. Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences , Tehran 1983969411 , Iran

5. Department of Microbiology and Virology, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran

6. Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz 5166616471 , Iran

Abstract

Abstract The antibacterial, antibiofilm, and cytotoxicity activity of cell-free supernatants (CFSs) from probiotics, including Lactobacillus plantarum, Bifidobacterium bifidum, and Saccharomyces cerevisiae against multi-drug resistant Escherichia coli evaluated in current research. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the CFSs were determined by analyzing inhibition zone formation using agar disk diffusion for antibacterial activity, microtiter plate for biofilm analysis, and auto-aggregation were done. CFSs substances were analyzed by GC-MS. The MTT assay on HEK293 cells investigated CFS’s influence on cell viability. CFSs were examined for biofilm-related virulence genes, including aggR and fimH using real-time polymerase chain reaction (real-time PCR). All CFSs had bacteriostatic and bactericidal effects. The B. bifidum exhibited the highest antibiofilm activity compared to the others. Bifidobacterium bifidum, L. plantarum, and S. cerevisiae produce 19, 16, and 11 mm inhibition zones against E. coli, respectively. GC-MS indicated that Hydroxyacetone, 3-Hydroxybutyric acid, and Oxime-methoxy-phenyl-dominated CFSs from L. plantarum, B. bifidum, and S. cerevisiae CFSs, respectively. The MTT test demonstrated a cell viability rate of over 90%. Statistically, adding all CFSs lowered the relative expression of both aggR and fimH virulence genes.

Funder

Tabriz University of Medical Sciences

Publisher

Oxford University Press (OUP)

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