<i>Pseudomonas aeruginosa</i> biofilm killing beyond the spacer by antibiotic-loaded calcium sulfate beads: an in vitro study
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Published:2021-03-23
Issue:5
Volume:6
Page:119-129
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ISSN:2206-3552
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Container-title:Journal of Bone and Joint Infection
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language:en
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Short-container-title:J. Bone Joint Infect.
Author:
Brooks Jacob R.,Dusane Devendra H.,Moore Kelly,Gupta Tripti,Delury Craig,Aiken Sean S.,Laycock Phillip A.,Sullivan Anne C.,Granger Jeffrey F.,Dipane Matthew V.,McPherson Edward J.,Stoodley Paul
Abstract
Abstract. Introduction: Bacterial biofilms are an important virulence factor in
chronic periprosthetic joint infection (PJI) and other orthopedic infection
since they are highly tolerant to antibiotics and host immunity. Antibiotics
are mixed into carriers such as bone cement and calcium sulfate bone void
fillers to achieve sustained high concentrations of antibiotics required to
more effectively manage biofilm infections through local release. The effect
of antibiotic diffusion from antibiotic-loaded calcium sulfate beads
(ALCS-B) in combination with PMMA bone cement spacers on the spread and
killing of Pseudomonas aeruginosa Xen41 (PA-Xen41) biofilm was investigated using a “large agar
plate” model scaled for clinical relevance. Methods: Bioluminescent
PA-Xen41 biofilms grown on discs of various orthopedic materials were placed within a large agar plate containing a PMMA full-size mock “spacer”
unloaded or loaded with vancomycin and tobramycin, with or without ALCS-B.
The amount of biofilm spread and log reduction on discs at varying distances
from the spacer was assessed by bioluminescent imaging and viable cell
counts. Results: For the unloaded spacer control, PA-Xen41 spread from the
biofilm to cover the entire plate. The loaded spacer generated a 3 cm zone of
inhibition and significantly reduced biofilm bacteria on the discs
immediately adjacent to the spacer but low or zero reductions on those further away. The combination of ALCS-B and a loaded PMMA spacer greatly
reduced bacterial spread and resulted in significantly greater biofilm
reductions on discs at all distances from the spacer. Discussion: The
addition of ALCS-B to an antibiotic-loaded spacer mimic increased the area of antibiotic coverage and efficacy against biofilm, suggesting that a
combination of these depots may provide greater physical antibiotic coverage
and more effective dead space management, particularly in zones where the
spread of antibiotic is limited by diffusion (zones with little or no fluid
motion).
Publisher
Copernicus GmbH
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