Genetic variation in the ovine KAP22-1 gene and its effect on wool traits in Egyptian sheep
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Published:2022-08-05
Issue:3
Volume:65
Page:293-300
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Sallam Ahmed M.ORCID, Gad-Allah Aymen A., Albetar Essam M.
Abstract
Abstract. The objective of this study was to investigate the genetic polymorphisms in
the keratin-associated protein (KAP22-1) gene in Barki (n=206), Rahmani (n=28)
and Ossimi (n=28) as the three major sheep breeds in Egypt. Subsequently,
the detected variants were correlated with important wool traits. The traits
included greasy fleece weight (GFW, g), staple length (SL, cm), prickle
factor (PF, %), medullated fiber (MF, %), fiber diameter (FD, µm), crimp percentage (CR, %) and the standard deviation of FD
(SDfd, µm), as well as the subjectively assessed traits of
kemp score (KS), handle grade (HG), greasy color grade (GCG), bulk grade
(BG), luster grade (LG) and staple structure (SST). Animals were
genotyped by polymerase chain reaction (PCR) – single strand conformation
polymorphism (SSCP). Five SSCP banding patterns representing three different
nucleotide variants (A, B and C) were detected. DNA sequencing confirmed
three single nucleotide polymorphisms (SNPs). Animal age significantly
affected GFW (P=0.007), SDfd (P=0.006), SL (P=0.002), CR
(P=0.006), KS (P=0.001), LG (P=0.006) and SST (P=0.013). Likewise, the
breed had a significant effect on all studied traits except HG and BG, which
was not significant. Results showed significant associations between the
KAP22-1 variants and CR (P=0.01), SL (P=0.012), KS (P<0.001) and GCG
(P=0.01). Interestingly, animals with BB genotypes tended to produce more
wool yield (1163.63±65.91 g) with high SL (8.38±0.20 cm), CR
(8.38±0.21 %) and KS (1.98±1.88). Results of this study
strongly recommend the KAP22-1 gene as a candidate gene for wool production traits
in Egyptian sheep, with new useful insights into the visually assessed wool
traits. The identified genetic markers may be incorporated into breeding
strategies and genetic improvement programs of wool traits in Egyptian
sheep.
Publisher
Copernicus GmbH
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