Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens
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Published:2022-08-03
Issue:3
Volume:65
Page:275-284
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Chen Chuwen, Li Jie, Li ZhixiongORCID
Abstract
Abstract. To investigate the effects of miR-499-5p on muscle
metabolism in broiler chickens, eight broiler chicks were assigned to the
control group and eight to the treatment group, and then we monitored the effects
using metabolomics. Chicks were fed basal diets without or with miR-499-5p
delivery. Gastrocnemius muscle samples were collected and analyzed by
ultrahigh-performance liquid chromatography–tandem mass spectrometry. The
results showed that miR-499-5p injection altered the concentrations of a
variety of metabolites in the gastrocnemius muscle. Thereby, a total of 46
metabolites were identified at higher (P<0.05) concentrations and
30 metabolites were identified at lower (P<0.05) concentrations in
the treatment group compared with the control group. These metabolites
were primarily involved with the regulation of lipid and carbohydrate
metabolism. Further metabolic pathway analysis revealed that fructose and
mannose metabolism, galactose metabolism, inositol phosphate metabolism, and
terpenoid backbone biosynthesis were the most critical pathway which may
partially interpret the effects of miR-499-5p. To our knowledge, this
research is the first report of metabolic signatures and related metabolic
pathways in the skeletal muscle for miR-499-5p injection and provides new
insight into the effect of miRNA on growth performance.
Funder
Sichuan Province Science and Technology Support Program
Publisher
Copernicus GmbH
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