Genetic polymorphism of the ovine MAP3K5 gene and its association with body size traits in Hu sheep of China
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Published:2023-02-16
Issue:1
Volume:66
Page:71-79
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Yang Xiaobin,Wang Weimin,Zhang Deyin,Li Xiaolong,Zhang Yukun,Zhao Yuan,Zhao Liming,Wang Jianghui,Xu Dan,Cheng Jiangbo,Li Wenxin,Zhou Bubo,Lin Changchun,Zeng Xiwen,Zhai Rui,Ma Zongwu,Liu Jia,Cui Panpan,Zhang Xiaoxue
Abstract
Abstract. As an excellent local sheep breed in China, Hu sheep have the
characteristics of producing more lambs and good motherhood. The purpose of
this study was to identify the polymorphism of the mitogen-activated protein
kinase 5 (MAP3K5) gene and determine whether it was associated with the body size
traits (body height, body length, chest circumference, and cannon
circumference) in Hu sheep. The polymorphism of MAP3K5 was identified by using PCR
amplification, followed by Sanger sequencing, and KASPar (Kompetitive Allele Specific PCR) technology was used for genotyping subsequently. The results of the association analysis showed
that MAP3K5 (g.205261 A > G) was significantly associated with body
height at 80, 100, 140, 160, and 180 d; body length at 80 and 160 d;
chest circumference at 100, 140, 160, and 180 d; and cannon circumference at
100 and 120 d, respectively. The results of qRT-PCR showed that the
expression level of MAP3K5 in the heart was significantly higher (P < 0.05)
than in the other 10 tissues. In summary, the MAP3K5 mutation loci may be used as
a candidate molecular marker for the body size traits of Hu sheep.
Funder
National Natural Science Foundation of China Gansu Agricultural University
Publisher
Copernicus GmbH
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