Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia
-
Published:2018-12-19
Issue:4
Volume:61
Page:481-489
-
ISSN:2363-9822
-
Container-title:Archives Animal Breeding
-
language:en
-
Short-container-title:Arch. Anim. Breed.
Author:
Amiri Sihem, Jemmali BayremORCID, Ferchichi Mohamed Amine, Jeljeli Hajer, Boulbaba Rekik, Ben Gara Abderrahmane
Abstract
Abstract. Research to assess the effect of single genes on reproductive traits in
bovine species is imperative to elucidate genes' functions and acquire a
better perspective of quantitative traits. The present study was undertaken
to characterize genetic diversity in the bovine growth hormone (GH) gene in a
population of 410 Holstein dairy cows in Tunisia. The analyses were based on
single nucleotide polymorphisms, and GH-AluI and GH-MspI detections and genotyping
were carried out using the polymerase chain reaction-restriction
fragment length polymorphism (PCR-RFLP) method. Data were analyzed using a mixed linear model with the MIXED procedure
to reveal the possible effect of GH genotypes on reproductive traits.
The frequency data of AluI(L//V) and MspI(+//−)
alleles were 87.04//12.96 and 70.06//29.94, respectively.
The distribution of the frequency of GH
genotypes for LL/LV/VV and (−/−)//(+/−)//(+/+) were
77.75//18.59//3.66 and 15.37//29.13//55.50, respectively. The results of the
statistical analyses proved that GH-AluI showed a substantial favorable
effect on exanimate traits except for the age at first calving; however, only
a suggestive effect of GH-MspI on the calving interval (CI) and the days open (DI) was
found. The homozygous LL genotype seemed to be advantageous with respect to the
CI and the DI compared with LV and VV genotypes. Heterozygous MspI(+/−) cows
tended to have a longer CI and DI than MspI(+/+) and
MspI(−/−) cows, but the difference was not statistically significant.
A significant effect of different GH-AluI–MspI combined genotypes was
found on the number of inseminations per conception, the CI and
the DI, and the LL/– combined genotype seemed to be associated with
better reproductive performance.
Based on these results, the LL genotype of the GH locus can be
considered to be a favorable genotype for reproductive traits in Holstein dairy
cattle, although these findings need to be confirmed by further research before
polymorphisms can be used in a marker-assisted selection program.
Publisher
Copernicus GmbH
Reference40 articles.
1. Akers, R. M.: Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows, J. Dairy Sci., 89, 1222–1234, 2006. 2. Anggraeni, A., Talib, C., Asmarasari, S. A., Herawati, T., and Andreas, E.: Genetic Polymorphisms of IGF1, GH, and OPN Genes in Crosses Peranakan Ongole Cattle Based on Birth Type in Central Java, JITV, 22, 165–172, 2017. 3. Arango, J. G., Echeverri, Z. J., and López, H. A.: Association of the bovine growth hormone gene with Holstein cattle reproductive parameters, Rev. MVZ Córdoba 19, 4249–4258, 2014. 4. Aruna, P., Chakravarty, A. K., Bhattacharya, T. K., Joshi, B. K., and Arjava, S.: Detection of Polymorphism of Growth Hormone Gene for the Analysis of Relationship between Allele Type and Growth Traits in Karan Fries Cattle, Asian-Aust, J. Anim. Sci. 17, 1334–1337, 2004. 5. Balogh, O., Kovács, K., Kulcsár, M., Gáspárdy, A., Fébel, H., Zsolnai, A., Fésus, C., Delavaud, C., Chilliard, Y., Gilbert, R., and Huszenicza, G.: Interrelationship of growth hormone AluI polymorphism and hyperketonemia with plasma hormones and metabolites in the beginning of lactation in dairy cows, Livest. Sci., 123, 180–186, 2009a.
Cited by
18 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|