Do bacterial viruses affect framboid-like mineral formation?

Abstract

Abstract. Framboidal pyrite has been a matter of interest of many studies due to its abundance in a wide range of environments and being a marker of redox conditions. However, the clear origin of framboidal pyrite remains unresolved. Our studies are preliminary laboratory investigations on the influence of the shape and physicochemical properties of bacteriophages on the synthesis of framboid-like structures. This paper discusses the possible role of bacteriophages (bacterial viruses) in the precipitation of sulfide minerals (FeS and CuS) and their impact on the formation of framboid-like structures. Here, two bacteriophages (Escherichia phage P1 and Pseudomonas phage Φ6), which differ significantly in shape and physicochemical properties, were used. Our observations suggest that viruses may bind ions from the solution. Moreover, we showed that bacteriophages P1 can lead to the formation of finer mineral particles of FeS and CuS, whereas the framboid-like structures were found only in experiments with precipitation of FeS. However, the lipid-enveloped Pseudomonas phage Φ6 did not cause the formation of similar structures. It is assumed that Escherichia phage P1 can promote the formation of FeS-based framboid-like or spherical structures. The proposed four-step conceptualized mechanism facilitating the framboid-like structure synthesis via viruses is as follows: (i) binding of ions by capsids, (ii) bacteriophages behaving like a crystallization surface, (iii) destabilization of the colloid (ζ potential ± 0), and (iv) formation of fine agglomerates and subsequent formation of small crystallites. Further studies are required to find all factors that may be affected by bacteriophages during sulfide precipitation. In addition, it is important to consider viruses present in sedimentation environments, despite possible difficulties in laboratory culturing. The consideration of such viruses may make laboratory testing more valid in terms of sedimentation environments.