Abstract
Abstract. Forest biodiversity is essential in maintaining ecosystem functions and services. Recently, unmanned aerial vehicle (UAV) remote sensing technology has emerged as a cost-effective and flexible tool for biodiversity monitoring. In this study, we compared the optimal clustering algorithm, classification method (spectral angle mapper, SAM), spectral diversity metric and structural heterogeneity index for forest species diversity estimation in two complex subtropical forests, Mazongling (MZL) and Gonggashan (GGS) National Nature Forest Reserves in China, using UAV-borne hyperspectral and LiDAR data. The results showed that the SAM classification method performed better with higher values of R2 than the clustering algorithm for predicting both species richness (MZL: 0.62 > 0.46 and GGS: 0.55 > 0.46) and Shannon-Wiener index (MZL: 0.64 > 0.58 and GGS: 0.52 > 0.47), while the optimal clustering algorithm had the highest prediction accuracy for the Simpson index, followed by the SAM classification method, spectral diversity metric and structural heterogeneity index (MZL: 0.83>0.44>0.31>0.12, GGS: 0.62>0.44>0.38>0.00). Our study indicated that the SAM classification method had the advantage of identifying rare species and estimating species richness, while the clustering method could capture forest diversity patterns rapidly without distinguishing the specific tree species and predict the Simpson index more accurately. Overall, both clustering and classification methods exhibited superior performance compared to spectral or structural diversity indices. Our findings highlight the applicability of UAV remote sensing in monitoring forest species diversity in complex subtropical forests.