The role of miR-369-3p in proliferation and differentiation of preadipocytes in Aohan fine-wool sheep-Reference-Cited by-同舟云学术

The role of miR-369-3p in proliferation and differentiation of preadipocytes in Aohan fine-wool sheep

Published:2023-02-27 Issue:1 Volume:66 Page:93-102
ISSN:2363-9822
Container-title:Archives Animal Breeding
language:en
Short-container-title:Arch. Anim. Breed.

Author:

Xue Shijie,Liu Kaidong,Zhao Le,Zhou Lisheng,Gao Xiaoxiao,Liu Lirong,Liu Nan,He Jianning

Abstract

Abstract. MicroRNAs (miRNAs) are a large class of non-coding RNAs that play important roles in the proliferation and differentiation of adipocytes. Our previous sequencing analysis revealed higher expression of miR-369-3p in the longissimus muscle of 2-month-old Aohan fine-wool sheep (AFWS) compared to 12-month-old sheep (P<0.05), suggesting that miR-369-3p may regulate fat deposition in AFWS. To test this, miR-369-3p mimics, inhibitors, and negative controls (NCs) were constructed and transfected into AFWS preadipocytes. After transfection with miR-369-3p mimics, we found a decrease (P<0.05) in the expression of genes and proteins related to cell proliferation and differentiation, detected by RT-qPCR (quantitative reverse transcription PCR) and western blot analyses. Moreover, EdU (5-ethynyl-2′-deoxyuridine) detection and Oil Red O staining showed a decrease (P<0.05) in cell proliferation and lipid accumulation, respectively. The opposite trends (P<0.05) were obtained after transfection with miR-369-3p inhibitors. In conclusion, the results showed that miR-369-3p can inhibit the proliferation and differentiation of AFWS preadipocytes, providing a theoretical basis to further explore the molecular mechanism of fat deposition in sheep and other domestic animals.

Funder

Natural Science Foundation of Shandong Province

Agricultural Variety Improvement Project of Shandong Province

Publisher

Copernicus GmbH

Subject

General Medicine

Link

https://aab.copernicus.org/articles/66/93/2023/aab-66-93-2023.pdf

Reference42 articles.

1. An, H. X., Beckmann, M. W., Reifenberger, G., Bender, H. G., and Niederacher, D.: Gene amplification and overexpression of CDK4 in sporadic breast carcinomas is associated with high tumor cell proliferation, Am. J. Pathol., 154, 113–118, https://doi.org/10.1016/s0002-9440(10)65257-1, 1999.

2. Arner, P. and Kulyté, A.: MicroRNA regulatory networks in human adipose tissue and obesity, Nat. Rev. Endocrinol., 11, 276–288, https://doi.org/10.1038/nrendo.2015.25, 2015.

3. Azad, T., Tashakor, A., and Hosseinkhani, S.: Split-luciferase complementary assay: applications, recent developments, and future perspectives, Anal. Bioanal. Chem., 406, 5541–5560, https://doi.org/10.1007/s00216-014-7980-8, 2014.

4. Barak, Y., Nelson, M. C., Ong, E. S., Jones, Y. Z., Ruiz-Lozano, P., Chien, K. R., Koder, A., and Evans, R. M.: PPAR gamma is required for placental, cardiac, and adipose tissue development, Mol. Cell, 4, 585–595, https://doi.org/10.1016/s1097-2765(00)80209-9, 1999.

5. Blanchet, E., Annicotte, J. S., Lagarrigue, S., Aguilar, V., Clapé, C., Chavey, C., Fritz, V., Casas, F., Apparailly, F., Auwerx, J., and Fajas, L.: E2F transcription factor-1 regulates oxidative metabolism, Nat. Cell Biol., 13, 1146–1152, https://doi.org/10.1038/ncb2309, 2011.